Research Output 1980 2019

2019
2 Citations (Scopus)

Caffeine inhibits PI3K and mTORC2 in Dictyostelium and differentially affects multiple other cAMP chemoattractant signaling effectors

Tariqul Islam, A. F. M., Scavello, M., Lotfi, P., Daniel, D., Haldeman, P. & Charest, P. G., Jan 1 2019, In : Molecular and Cellular Biochemistry.

Research output: Contribution to journalArticle

Phosphatidylinositol 3-Kinase
Dictyostelium
Chemotactic Factors
Caffeine
TOR complex 2
3 Citations (Scopus)

Endonuclease activity inhibition of the NS1 protein of parvovirus B19 as a novel target for antiviral drug development

Xu, P., Ganaie, S. S., Wang, X., Wang, Z., Kleiboeker, S., Horton, N. C., Heier, R. F., Meyers, M. J., Tavis, J. E. & Qiu, J., Mar 1 2019, In : Antimicrobial Agents and Chemotherapy. e01879-18.

Research output: Contribution to journalArticle

Endonucleases
Antiviral Agents
DNA Replication
Replication Origin
Terminal Repeat Sequences
2 Citations (Scopus)

Mechanism of Filamentation-Induced Allosteric Activation of the SgrAI Endonuclease

Polley, S., Lyumkis, D. & Horton, N. C., Oct 1 2019, In : Structure. 27, 10, p. 1497-1507.e3

Research output: Contribution to journalArticle

Open Access
Type II Site Specific Deoxyribonucleases
DNA Cleavage
DNA
Enzymes
endodeoxyribonuclease SgrAI

Novel insights into filament-forming enzymes

Park, C. K. & Horton, N. C., Jan 1 2019, (Accepted/In press) In : Nature Reviews Molecular Cell Biology.

Research output: Contribution to journalComment/debate

Enzymes
2 Citations (Scopus)

Structures, functions, and mechanisms of filament forming enzymes: a renaissance of enzyme filamentation

Park, C. K. & Horton, N. C., Dec 1 2019, In : Biophysical Reviews. 11, 6, p. 927-994 68 p.

Research output: Contribution to journalReview article

Open Access
Enzymes
Cellular Structures
Renaissance
In Vitro Techniques
3 Citations (Scopus)

The Need for Speed: Run-On Oligomer Filament Formation Provides Maximum Speed with Maximum Sequestration of Activity

Barahona, C. J., Basantes, L. E., Tompkins, K. J., Heitman, D. M., Chukwu, B. I., Sanchez, J., Sanchez, J. L., Ghadirian, N., Park, C. K. & Horton, N. C., Mar 1 2019, In : Journal of virology. 93, 5

Research output: Contribution to journalArticle

DNA Cleavage
Enzymes
enzymes
DNA
Virus Diseases
2018
1 Citation (Scopus)

The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a negative effect of caffeine and potential role of microtubules

Tariqul Islam, A. F. M., Yue, H., Scavello, M., Haldeman, P., Rappel, W. J. & Charest, P. G., Aug 1 2018, In : Cellular Signalling. 48, p. 25-37 13 p.

Research output: Contribution to journalArticle

Dictyostelium
Energy Transfer
Protein Subunits
Caffeine
GTP-Binding Proteins
4 Citations (Scopus)

The run-on oligomer filament enzyme mechanism of SgrAI: Part 2. Kinetic modeling of the full DNA cleavage pathway

Park, C. K., Sanchez, J. L., Barahona, C., Basantes, L. E., Sanchez, J., Hernandez, C. & Horton, N. C., Jan 1 2018, In : Journal of Biological Chemistry. 293, 38, p. 14599-14615 17 p.

Research output: Contribution to journalArticle

DNA Cleavage
Oligomers
Kinetics
DNA
Type II Site Specific Deoxyribonucleases
4 Citations (Scopus)

The run-on oligomer filament enzyme mechanism of SgrAI: Part 1. Assembly kinetics of the run-on oligomer filament

Park, C. K., Sanchez, J. L., Barahona, C., Basantes, L. E., Sanchez, J., Hernandez, C. & Horton, N. C., Jan 1 2018, In : Journal of Biological Chemistry. 293, 38, p. 14585-14598 14 p.

Research output: Contribution to journalArticle

DNA Cleavage
Oligomers
Kinetics
DNA
Enzymes
2017
7 Citations (Scopus)

Protein kinase A regulates the Ras, Rap1 and TORC2 pathways in response to the chemoattractant cAMP in Dictyostelium

Scavello, M., Petlick, A. R., Ramesh, R., Thompson, V. F., Lotfi, P. & Charest, P. G., May 1 2017, In : Journal of Cell Science. 130, 9, p. 1545-1558 14 p.

Research output: Contribution to journalArticle

Dictyostelium
Chemotactic Factors
Cyclic AMP-Dependent Protein Kinases
Signal Transduction
Chemotaxis
2016
5 Citations (Scopus)

DNA Binding and Cleavage by the Human Parvovirus B19 NS1 Nuclease Domain

Sanchez, J. L., Romero, Z., Quinones, A., Torgeson, K. R. & Horton, N. C., Nov 29 2016, In : Biochemistry. 55, 47, p. 6577-6593 17 p.

Research output: Contribution to journalArticle

Erythema Infectiosum
Human Parvovirus B19
DNA Cleavage
Replication Origin
Transcriptional Activation
1 Citation (Scopus)

Studying chemoattractant signal transduction dynamics in Dictyostelium by BRET

Islam, A. F. M. T., Stepanski, B. M. & Charest, P. G., 2016, Methods in Molecular Biology. Humana Press Inc., Vol. 1407. p. 63-77 15 p. (Methods in Molecular Biology; vol. 1407).

Research output: Chapter in Book/Report/Conference proceedingChapter

Dictyostelium
Energy Transfer
Chemotactic Factors
Signal Transduction
Amoeba
22 Citations (Scopus)

The small GTPases Ras and Rap1 bind to and control TORC2 activity

Khanna, A., Lotfi, P., Chavan, A. J., Montaño, N. M., Bolourani, P., Weeks, G., Shen, Z., Briggs, S. P., Pots, H., Van Haastert, P. J. M., Kortholt, A. & Charest, P. G., May 13 2016, In : Scientific Reports. 6, 25823.

Research output: Contribution to journalArticle

Monomeric GTP-Binding Proteins
Dictyostelium
Cell Movement
ras Proteins
TOR complex 2
2015
7 Citations (Scopus)

Probing the run-on oligomer of activated SgrAI bound to DNA

Shah, S., Sanchez, J., Stewart, A., Piperakis, M. M., Cosstick, R., Nichols, C., Park, C. K., Ma, X., Wysocki, V., Bitinaite, J. & Horton, N. C., Apr 16 2015, In : PLoS One. 10, 4, e0124783.

Research output: Contribution to journalArticle

Oligomers
DNA
Chemical activation
Oligomerization
Type II Site Specific Deoxyribonucleases

Structure and specificity of FEN-1 from Methanopyrus kandleri

Shah, S., Dunten, P., Stiteler, A., Park, C. K. & Horton, N. C., 2015, In : Proteins: Structure, Function and Genetics. 83, 1, p. 188-194 7 p.

Research output: Contribution to journalArticle

Flap Endonucleases
DNA Repair
Repair
Nucleotides
Genomic Instability
2014
2 Citations (Scopus)
Medical Students
Physiological Phenomena
Students
Physicians
Health Occupations
2013
14 Citations (Scopus)

Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization

Lyumkis, D., Talley, H., Stewart, A., Shah, S., Park, C. K., Tama, F., Potter, C. S., Carragher, B. & Horton, N. C., Oct 8 2013, In : Structure. 21, 10, p. 1848-1858 11 p.

Research output: Contribution to journalArticle

Allosteric Regulation
DNA Cleavage
DNA
Proteins
Enzyme Activation
11 Citations (Scopus)

Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility

Kölsch, V., Shen, Z., Lee, S., Plak, K., Lotfi, P., Chang, J., Charest, P. G., Romero, J. L., Jeon, T. J., Kortholt, A., Briggs, S. P. & Firtel, R. A., Jan 15 2013, In : Molecular Biology of the Cell. 24, 2, p. 100-114 15 p.

Research output: Contribution to journalArticle

Glycogen Synthase Kinase 3
Cell Movement
Myosin Type II
Chemotaxis
1-Phosphatidylinositol 4-Kinase
13 Citations (Scopus)

Structural analysis of activated SgrAI-DNA oligomers using ion mobility mass spectrometry

Ma, X., Shah, S., Zhou, M., Park, C. K., Wysocki, V. H. & Horton, N. C., Jun 25 2013, In : Biochemistry. 52, 25, p. 4373-4381 9 p.

Research output: Contribution to journalArticle

Oligomers
Structural analysis
Mass spectrometry
Mass Spectrometry
Ions
2011
8 Citations (Scopus)
DNA Cleavage
DNA
Type II Site Specific Deoxyribonucleases
Metals
Ions
2010
15 Citations (Scopus)

Activation of DNA cleavage by oligomerization of DNA-bound SgrAI

Park, C. K., Stiteler, A. P., Shah, S., Ghare, M. I., Bitinaite, J. & Horton, N. C., Oct 19 2010, In : Biochemistry. 49, 41, p. 8818-8830 13 p.

Research output: Contribution to journalArticle

Oligomerization
DNA Cleavage
Chemical activation
DNA
Molecular Weight
1 Citation (Scopus)

Crystallization of zinc finger proteins bound to DNA

Horton, N. C. & Park, C. K., 2010, Methods in Molecular Biology. Vol. 649. p. 457-477 21 p. (Methods in Molecular Biology; vol. 649).

Research output: Chapter in Book/Report/Conference proceedingChapter

Zinc Fingers
Crystallization
DNA
Proteins
13 Citations (Scopus)

Domain swapping in allosteric modulation of DNA specificity

Park, C. K., Joshi, H. K., Agrawal, A., Ghare, M. I., Little, E. J., Dunten, P. W., Bitinaite, J. & Horton, N. C., Dec 2010, In : PLoS Biology. 8, 12, e1000554.

Research output: Contribution to journalArticle

Modulation
DNA
Dimers
DNA Cleavage
restriction endonucleases
2009

The restriction enzyme SgrAI: Structure solution via combination of poor MIRAS and MR phases

Dunten, P. W., Little, E. J. & Horton, N. C., Mar 19 2009, In : Acta Crystallographica Section D: Biological Crystallography. 65, 4, p. 393-398 6 p.

Research output: Contribution to journalArticle

Type II Site Specific Deoxyribonucleases
Electrons
Enzymes
DNA
Datasets
2008
7 Citations (Scopus)

DNA Distortion and Specificity in a Sequence-Specific Endonuclease

Babic, A. C., Little, E. J., Manohar, V. M., Bitinaite, J. & Horton, N. C., Oct 31 2008, In : Journal of Molecular Biology. 383, 1, p. 186-204 19 p.

Research output: Contribution to journalArticle

Endonucleases
DNA
Enzymes
Nucleic Acid Conformation
DNA Cleavage
16 Citations (Scopus)

Early Interrogation and Recognition of DNA Sequence by Indirect Readout

Little, E. J., Babic, A. C. & Horton, N. C., Dec 12 2008, In : Structure. 16, 12, p. 1828-1837 10 p.

Research output: Contribution to journalArticle

Recombinational DNA Repair
DNA
Enzymes
Proteins
GTYRAC-specific type II deoxyribonucleases
23 Citations (Scopus)

The structure of SgrAI bound to DNA; recognition of an 8 base pair target

Dunten, P. W., Little, E. J., Gregory, M. T., Manohar, V. M., Dalton, M., Hough, D., Bitinaite, J. & Horton, N. C., 2008, In : Nucleic Acids Research. 36, 16, p. 5405-5416 12 p.

Research output: Contribution to journalArticle

Base Pairing
DNA
Type II Site Specific Deoxyribonucleases
Divalent Cations
DNA-Binding Proteins
2006
21 Citations (Scopus)

Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism

Joshi, H. K., Etzkorn, C., Chatwell, L., Bitinaite, J. & Horton, N. C., Aug 18 2006, In : Journal of Biological Chemistry. 281, 33, p. 23852-23869 18 p.

Research output: Contribution to journalArticle

Type II Site Specific Deoxyribonucleases
DNA
Mutation
Base Pairing
DNA-Binding Proteins
73 Citations (Scopus)

Structure of Aart, a Designed Six-finger Zinc Finger Peptide, Bound to DNA

Segal, D. J., Crotty, J. W., Bhakta, M. S., Barbas, C. F. & Horton, N. C., Oct 20 2006, In : Journal of Molecular Biology. 363, 2, p. 405-421 17 p.

Research output: Contribution to journalArticle

Zinc Fingers
Fingers
Peptides
DNA
Proteins
2005
5 Citations (Scopus)
Zinc Fingers
Crystallization
Fingers
peptides
Zinc
3 Citations (Scopus)

DNA-induced conformational changes in type II restriction endonucleases: The structure of unliganded HincII

Little, E. J. & Horton, N. C., Aug 5 2005, In : Journal of Molecular Biology. 351, 1, p. 76-88 13 p.

Research output: Contribution to journalArticle

Type II Site Specific Deoxyribonucleases
DNA
Endonucleases
2004
24 Citations (Scopus)

Ca2+ binding in the active site of HincII: Implications for the catalytic mechanism

Etzkorn, C. & Horton, N. C., Oct 26 2004, In : Biochemistry. 43, 42, p. 13256-13270 15 p.

Research output: Contribution to journalArticle

Catalytic Domain
Type II Site Specific Deoxyribonucleases
Phosphates
Ions
DNA Cleavage
76 Citations (Scopus)

DNA cleavage by EcoRV endonuclease: Two metal ions in three metal ion binding sites

Horton, N. C. & Perona, J. J., Jun 8 2004, In : Biochemistry. 43, 22, p. 6841-6857 17 p.

Research output: Contribution to journalArticle

DNA Cleavage
Metal ions
Metals
Phosphates
Binding Sites
14 Citations (Scopus)

Enhanced insulin action on glucose transport and insulin signaling in 7-day unweighted rat soleus muscle

O'Keefe, M. P., Perez, F. R., Sloniger, J. A., Tischler, M. E. & Henriksen, E. J., Jul 2004, In : Journal of Applied Physiology. 97, 1, p. 63-71 9 p.

Research output: Contribution to journalArticle

Hindlimb Suspension
Skeletal Muscle
Phosphatidylinositol 3-Kinase
Insulin
Glucose
16 Citations (Scopus)

Mechanistic insights from the structures of HincII bound to cognate DNA cleaved from addition of Mg 2+ and Mn 2+

Etzkorn, C. & Horton, N. C., Oct 29 2004, In : Journal of Molecular Biology. 343, 4, p. 833-849 17 p.

Research output: Contribution to journalArticle

Catalytic Domain
Phosphates
DNA
Hydrogen Bonding
Ions
2002
4 Citations (Scopus)

Differential half-maximal effects of human insulin and its analogs for in situ glucose transport and protein synthesis in rat soleus muscle

Weinstein, R. B., Eleid, N., LeCesne, C., Durando, B., Crawford, J. T., Heffner, M., Layton, C., O'Keefe, M., Robinson, J., Rudinsky, S., Henriksen, E. J. & Tischler, M. E., 2002, In : Metabolism: Clinical and Experimental. 51, 8, p. 1065-1070 6 p.

Research output: Contribution to journalArticle

Facilitative Glucose Transport Proteins
Skeletal Muscle
Insulin
Glucose
Proteins
21 Citations (Scopus)

Electrostatic contributions to site specific DNA cleavage by EcoRV endonuclease

Horton, N. C., Otey, C., Lusetti, S., Sam, M. D., Kohn, J., Martin, A. M., Ananthnarayan, V. & Perona, J. J., Sep 3 2002, In : Biochemistry. 41, 35, p. 10754-10763 10 p.

Research output: Contribution to journalArticle

DNA Cleavage
Static Electricity
Electrostatics
Catalytic Domain
DNA
48 Citations (Scopus)

Sequence selectivity and degeneracy of a restriction endonuclease mediated by DNA intercalation

Horton, N. C., Dorner, L. F. & Perona, J. J., 2002, In : Nature Structural Biology. 9, 1, p. 42-47 6 p.

Research output: Contribution to journalArticle

DNA Restriction Enzymes
Intercalation
DNA
Glutamine
Base Pairing
1997
5 Citations (Scopus)
Glucose Transporter Type 4
Citrate (si)-Synthase
Skeletal Muscle
Denervation
Muscle Denervation
8 Citations (Scopus)

Insulin attenuates atrophy of unweighted soleus muscle by amplified inhibition of protein degradation

Tischler, M. E., Satarug, S., Aannestad, A., Munoz, K. A. & Henriksen, E. J., 1997, In : Metabolism: Clinical and Experimental. 46, 6, p. 673-679 7 p.

Research output: Contribution to journalArticle

Proteolysis
Atrophy
Skeletal Muscle
Insulin
Muscles
5 Citations (Scopus)

Lysosomal proteolysis in distally or proximally denervated rat soleus muscle

Weinstein, R. B., Slentz, M. J., Webster, K., Takeuchi, J. A. & Tischler, M. E., 1997, In : American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 273, 4 42-4

Research output: Contribution to journalArticle

Denervation
Proteolysis
Skeletal Muscle
Atrophy
Muscles
1996
19 Citations (Scopus)

Are there oxygen-deficient regions in resting skeletal muscle?

Toth, A., Pal, M., Tischler, M. E. & Johnson, P. C., Jun 1996, In : American Journal of Physiology - Heart and Circulatory Physiology. 39, 6

Research output: Contribution to journalArticle

Skeletal Muscle
Fluorescence
Oxygen
Anaerobiosis
NAD
1995
5 Citations (Scopus)

Impact of weightlessness on muscle function.

Tischler, M. E. & Slentz, M., Oct 1995, In : ASGSB bulletin : publication of the American Society for Gravitational and Space Biology. 8, 2, p. 73-81 9 p.

Research output: Contribution to journalArticle

Weightlessness
Space Flight
Muscles
Muscle Proteins
Gravitation
5 Citations (Scopus)
arylphorins
Manduca
Manduca sexta
Phenylalanine
phenylalanine
1994
5 Citations (Scopus)

Cardiac protein content and synthesis in vivo after voluntary running or head-down suspension

Henriksen, E. J., Munoz, K. A., Aannestad, A. T. & Tischler, M. E., 1994, In : Journal of Applied Physiology. 76, 6, p. 2814-2819 6 p.

Research output: Contribution to journalArticle

Running
Suspensions
Head
Proteins
Head-Down Tilt
17 Citations (Scopus)
Skeletal Muscle
Glucocorticoids
Weight-Bearing
Hindlimb Suspension
Atrophy
108 Citations (Scopus)

Programmed cell death of the normal human neutrophil: An in vitro model of senescence

Payne, C. M., Glasser, L., Tischler, M. E., Wyckoff, D., Cromey, D., Fiederlein, R. & Bohnert, O., 1994, In : Microscopy Research and Technique. 28, 4, p. 327-344 18 p.

Research output: Contribution to journalArticle

neutrophils
apoptosis
Cell death
Neutrophils
Cell Death
18 Citations (Scopus)

Skeletal muscle protein content and synthesis after voluntary running and subsequent unweighting

Munoz, K. A., Aannestad, A., Tischler, M. E. & Henriksen, E. J., 1994, In : Metabolism: Clinical and Experimental. 43, 8, p. 994-999 6 p.

Research output: Contribution to journalArticle

Muscle Proteins
Running
Hindlimb Suspension
Skeletal Muscle
Proteins
1993
13 Citations (Scopus)

Elevated interstitial fluid volume in soleus muscles unweighted by spaceflight or suspension

Henriksen, E. J., Tischler, M. E., Woodman, C. R., Munoz, K. A., Stump, C. S. & Kirby, C. R., 1993, In : Journal of Applied Physiology. 75, 4, p. 1650-1653 4 p.

Research output: Contribution to journalArticle

Space Flight
Extracellular Fluid
Suspensions
Skeletal Muscle
Hindlimb Suspension
61 Citations (Scopus)

Spaceflight on STS-48 and earth-based unweighting produce similar effects on skeletal muscle of young rats

Tischler, M. E., Henriksen, E. J., Munoz, K. A., Stump, C. S., Woodman, C. R. & Kirby, C. R., 1993, In : Journal of Applied Physiology. 74, 5, p. 2161-2165 5 p.

Research output: Contribution to journalArticle

Space Flight
Animal Flight
Skeletal Muscle
Hindlimb Suspension
Muscles