Indraneel Ghosh

Article

A coiled-coil enabled split-luciferase three-hybrid system: Applied toward profiling inhibitors of protein kinases

Jester, B. W., Cox, K. J., Gaj, A., Shomin, C. D., Porter, J. R. & Ghosh, I., Aug 25 2010, In : Journal of the American Chemical Society. 132, 33, p. 11727-11735 9 p.

Research output: Contribution to journal › Article

Protein Kinase Inhibitors

Luciferases

Hybrid systems

Protein Kinases

Proteins

9 Citations (Scopus)

A comprehensive panel of turn-on caspase biosensors for investigating caspase specificity and caspase activation pathways

Shekhawat, S. S., Campbell, S. T. & Ghosh, I., Oct 17 2011, In : ChemBioChem. 12, 15, p. 2353-2364 12 p.

Research output: Contribution to journal › Article

Biosensing Techniques

Caspases

Biosensors

Chemical activation

Caspase 7

A fragment-based selection approach for the discovery of peptide macrocycles targeting protein kinases

Restituyo, E., Camacho-Soto, K. & Ghosh, I., 2015, In : Methods in molecular biology (Clifton, N.J.). 1248, p. 95-104 10 p.

Research output: Contribution to journal › Article

Protein Kinases

Cyclic Peptides

Peptides

Staurosporine

Bacteriophages

48 Citations (Scopus)

A general and rapid cell-free approach for the interrogation of protein-protein, protein-DNA, and protein-RNA interactions and their antagonists utilizing split-protein reporters

Porter, J. R., Stains, C. I., Jester, B. W. & Ghosh, I., May 21 2008, In : Journal of the American Chemical Society. 130, 20, p. 6488-6497 10 p.

Research output: Contribution to journal › Article

RNA

DNA

Proteins

Cell-Free System

Nucleic acids

21 Citations (Scopus)

A general approach for receptor and antibody-targeted detection of native proteins utilizing split-luciferase reassembly

Stains, C. I., Furman, J. L., Porter, J. R., Rajagopal, S., Li, Y., Wyatt, R. T. & Ghosh, I., Oct 15 2010, In : ACS Chemical Biology. 5, 10, p. 943-952 10 p.

Research output: Contribution to journal › Article

Luciferases

Antibodies

Single-Chain Antibodies

Proteins

Assays

12 Citations (Scopus)

A minimalist approach toward protein recognition by epitope transfer from functionally evolved β-sheet surfaces

Rajagopal, S., Meyer, S. C., Goldman, A., Zhou, M. & Ghosh, I., Nov 8 2006, In : Journal of the American Chemical Society. 128, 44, p. 14356-14363 8 p.

Research output: Contribution to journal › Article

Epitopes

Thrombin

Scaffolds

Proteins

Molecules

53 Citations (Scopus)

An autoinhibited coiled-coil design strategy for split-protein protease sensors

Shekhawat, S. S., Porter, J. R., Sriprasad, A. & Ghosh, I., Oct 28 2009, In : Journal of the American Chemical Society. 131, 42, p. 15284-15290 7 p.

Research output: Contribution to journal › Article

Peptide Hydrolases

Proteins

Sensors

Biosensing Techniques

Biosensors

357 Citations (Scopus)

Antiparallel leucine zipper-directed protein reassembly: Application to the green fluorescent protein [12]

Ghosh, I., Hamilton, A. D. & Regan, L., Jun 14 2000, In : Journal of the American Chemical Society. 122, 23, p. 5658-5659 2 p.

Research output: Contribution to journal › Article

Leucine Zippers

Fasteners

Green Fluorescent Proteins

Proteins

85 Citations (Scopus)

A pH-modulated, self-replicating peptide

Yao, S., Ghosh, I., Zutshi, R. & Chmielewski, J., 1997, In : Journal of the American Chemical Society. 119, 43, p. 10559-10560 2 p.

Research output: Contribution to journal › Article

Peptides

59 Citations (Scopus)

A self-replicating peptide under ionic control

Yao, S., Ghosh, I., Zutshi, R. & Chmielewski, J., Mar 2 1998, In : Angewandte Chemie - International Edition. 37, 4, p. 478-481 4 p.

Research output: Contribution to journal › Article

Peptides

4 Citations (Scopus)

A turn-on split-luciferase sensor for the direct detection of poly(ADP-ribose) as a marker for DNA repair and cell death

Furman, J. L., Mok, P. W., Shen, S., Stains, C. I. & Ghosh, I., Jan 7 2011, In : Chemical Communications. 47, 1, p. 397-399 3 p.

Research output: Contribution to journal › Article

Poly Adenosine Diphosphate Ribose

Firefly Luciferases

Administrative data processing

Cell death

Luciferases

22 Citations (Scopus)

A β-sheet peptide inhibitor of E47 dimerization and DNA binding

Ghosh, I. & Chmielewski, J., Aug 1998, In : Chemistry and Biology. 5, 8, p. 439-445 7 p.

Research output: Contribution to journal › Article

Dimerization

Basic Helix-Loop-Helix Transcription Factors

Transcription Factors

Peptides

DNA

10 Citations (Scopus)

Basic-helix-loop-helix region of tal: Evaluation of structure and DNA affinity

Bishop, P., Ghosh, I., Jones, C. & Chmielewski, J., Aug 9 1995, In : Journal of the American Chemical Society. 117, 31, p. 8283-8284 2 p.

Research output: Contribution to journal › Article

Basic–Helix–Loop–Helix Region of Tal: Evaluation of Structure and DNA Affinity

Bishop, P., Ghosh, I., Jones, C. & Chmielewski, J., 1995, In : Journal of the American Chemical Society. 117, 46, p. 11616 1 p.

Research output: Contribution to journal › Article

DNA

90 Citations (Scopus)

Current trends in peptide science quantum dots and peptides: A bright future together

Zhou, M. & Ghosh, I., 2007, In : Biopolymers - Peptide Science Section. 88, 3, p. 325-339 15 p.

Research output: Contribution to journal › Article

Quantum Dots

Peptides

Semiconductor quantum dots

Ligands

peptide A

301 Citations (Scopus)

Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: Scope and mechanism

Magliery, T. J., Wilson, C. G. M., Pan, W., Mishler, D., Ghosh, I., Hamilton, A. D. & Regan, L., Jan 12 2005, In : Journal of the American Chemical Society. 127, 1, p. 146-157 12 p.

Research output: Contribution to journal › Article

Green Fluorescent Proteins

Proteins

Proteomics

Leucine Zippers

Protein Binding

76 Citations (Scopus)

Direct detection of double-stranded DNA: Molecular methods and applications for DNA diagnostics

Ghosh, I., Stains, C. I., Ooi, A. T. & Segal, D. J., 2006, In : Molecular BioSystems. 2, 11, p. 551-560 10 p.

Research output: Contribution to journal › Article

Zinc Fingers

Zinc

Denaturation

Molecular Pathology

DNA

49 Citations (Scopus)

Direct DNA methylation profiling using methyl binding domain proteins

Yu, Y., Blair, S., Gillespie, D., Jensen, R., Myszka, D., Badran, A. H., Ghosh, I. & Chagovetz, A., Jun 15 2010, In : Analytical Chemistry. 82, 12, p. 5012-5019 8 p.

Research output: Contribution to journal › Article

Methylation

DNA Fingerprinting

DNA Methylation

Carrier Proteins

Proteins

1 Citation (Scopus)

DNA binding properties of basic helix-loop-helix fusion proteins of Tal and E47

Ghosh, I., Bishop, P. & Chmielewski, J., 2001, In : Journal of Peptide Research. 57, 5, p. 354-360 7 p.

Research output: Contribution to journal › Article

Fusion reactions

DNA

Helix-Loop-Helix Motifs

Basic Helix-Loop-Helix Transcription Factors

Proteins

66 Citations (Scopus)

DNA sequence-enabled reassembly of the green fluorescent protein

Stains, C. I., Porter, J. R., Ooi, A. T., Segal, D. J. & Ghosh, I., Aug 10 2005, In : Journal of the American Chemical Society. 127, 31, p. 10782-10783 2 p.

Research output: Contribution to journal › Article

DNA sequences

Green Fluorescent Proteins

Zinc Fingers

Proteins

Zinc

7 Citations (Scopus)

Dual surface selection methodology for the identification of thrombin binding epitopes from hotspot biased phage-display libraries

Rajagopal, S., Meza-Romero, R. & Ghosh, I., Mar 22 2004, In : Bioorganic and Medicinal Chemistry Letters. 14, 6, p. 1389-1393 5 p.

Research output: Contribution to journal › Article

Bacteriophages

Thrombin

Libraries

Epitopes

Immunoglobulin G

22 Citations (Scopus)

Evaluating the global CpG methylation status of native DNA utilizing a bipartite split-luciferase sensor

Badran, A. H., Furman, J. L., Ma, A. S., Comi, T. J., Porter, J. R. & Ghosh, I., Sep 15 2011, In : Analytical Chemistry. 83, 18, p. 7151-7157 7 p.

Research output: Contribution to journal › Article

Methylation

Luciferases

decitabine

DNA

Sensors

86 Citations (Scopus)

Helical Supramolecules and Fibers Utilizing Leucine Zipper-Displaying Dendrimers

Zhou, M., Bentley, D. & Ghosh, I., Jan 28 2004, In : Journal of the American Chemical Society. 126, 3, p. 734-735 2 p.

Research output: Contribution to journal › Article

Dendrimers

Leucine Zippers

Fasteners

Fibers

Nanotechnology

32 Citations (Scopus)

Highly selective cyclic peptide ligands for neutravidin and avidin identified by phage display

Meyer, S. C., Gaj, T. & Ghosh, I., Jul 2006, In : Chemical Biology and Drug Design. 68, 1, p. 3-10 8 p.

Research output: Contribution to journal › Article

Cyclic Peptides

Bacteriophages

Streptavidin

Avidin

Display devices

60 Citations (Scopus)

High specificity in protein recognition by hydrogen-bond-surrogate α-helices: Selective inhibition of the p53/MDM2 complex

Henchey, L. K., Porter, J. R., Ghosh, I. & Arora, P. S., Oct 18 2010, In : ChemBioChem. 11, 15, p. 2104-2107 4 p.

Research output: Contribution to journal › Article

Proto-Oncogene Proteins c-mdm2

Peptidomimetics

Tumor Suppressor Protein p53

Molecular recognition

X ray crystallography

98 Citations (Scopus)

Inhibition of spontaneous receptor phosphorylation by residues in a putative α-helix in the KIT intracellular juxtamembrane region

Ma, Y., Cunningham, M. E., Wang, X., Ghosh, I., Regan, L. & Longley, B. J., May 7 1999, In : Journal of Biological Chemistry. 274, 19, p. 13399-13402 4 p.

Research output: Contribution to journal › Article

Phosphorylation

Substitution reactions

Alanine

Conformations

Phosphotransferases

38 Citations (Scopus)

Inhibition of β-amyloid fibrillization by directed evolution of a β-sheet presenting miniature protein

Smith, T. J., Stains, C. I., Meyer, S. C. & Ghosh, I., Nov 15 2006, In : Journal of the American Chemical Society. 128, 45, p. 14456-14457 2 p.

Research output: Contribution to journal › Article

Amyloid

Proteins

Protein Transport

Circular dichroism spectroscopy

Denaturation

16 Citations (Scopus)

Ligand-gated split-kinases

Camacho-Soto, K., Castillo-Montoya, J., Tye, B. & Ghosh, I., Mar 12 2014, In : Journal of the American Chemical Society. 136, 10, p. 3995-4002 8 p.

Research output: Contribution to journal › Article

Protein Kinases

Phosphotransferases

Ligands

Proteins

Dissection

5 Citations (Scopus)

Mapping the BH3 binding interface of Bcl-x<inf>L</inf>, Bcl-2, and Mcl-1 using split-luciferase reassembly

Campbell, S. T., Carlson, K. J., Buchholz, C. J., Helmers, M. R. & Ghosh, I., Apr 28 2015, In : Biochemistry. 54, 16, p. 2632-2643 12 p.

Research output: Contribution to journal › Article

Luciferases

Alanine

Apoptosis

Mutation

Puma

83 Citations (Scopus)

Molecules that target beta-amyloid

Stains, C. I., Mondal, K. & Ghosh, I., Dec 10 2007, In : ChemMedChem. 2, 12, p. 1674-1692 19 p.

Research output: Contribution to journal › Article

Amyloid beta-Peptides

Amyloid

Molecules

Agglomeration

Peptidomimetics

77 Citations (Scopus)

New directions in targeting protein kinases: Focusing upon true allosteric and bivalent inhibitors

Lamba, V. & Ghosh, I., Jul 2012, In : Current Pharmaceutical Design. 18, 20, p. 2936-2945 10 p.

Research output: Contribution to journal › Article

Protein Kinases

Adenosine Triphosphate

Protein Kinase Inhibitors

Polypharmacology

Sequence Homology

15 Citations (Scopus)

Noncovalent multivalent assembly of jun peptides on a leucine zipper dendrimer displaying fos peptides

Zhou, M. & Ghosh, I., Sep 30 2004, In : Organic Letters. 6, 20, p. 3561-3564 4 p.

Research output: Contribution to journal › Article

zippers

Dendrimers

Leucine Zippers

leucine

dendrimers

2 Citations (Scopus)

Parsing the motion of single molecules: a novel algorithm for deconvoluting the dynamics of individual receptors at the cell surface.

Ghosh, I. & Wirth, M. J., Jun 16 2007, In : Science's STKE : signal transduction knowledge environment. 2007, 388

Research output: Contribution to journal › Article

Cell Surface Receptors

Brownian movement

Molecules

Biophysics

Signal transduction

88 Citations (Scopus)

Peptide-labeled quantum dots for imaging GPCRs in whole cells and as single molecules

Zhou, M., Nakatani, E., Gronenberg, L. S., Tokimoto, T., Wirth, M. J., Hruby, V. J., Roberts, A., Lynch, R. & Ghosh, I., Mar 2007, In : Bioconjugate Chemistry. 18, 2, p. 323-332 10 p.

Research output: Contribution to journal › Article

Quantum Dots

GTP-Binding Proteins

Peptides

Semiconductor quantum dots

Proteins

32 Citations (Scopus)

Peptide self-assembly as a model of proteins in the pre-genomic world

Ghosh, I. & Chmielewski, J., Dec 2004, In : Current Opinion in Chemical Biology. 8, 6, p. 640-644 5 p.

Research output: Contribution to journal › Article

Self assembly

Peptides

Nucleotide Aptamers

Catalytic RNA

Proteins

15 Citations (Scopus)

Probing orientations of single fluorescent labels on a peptide reversibly binding to the human delta-opioid receptor

Tokimoto, T., Bethea, T. R. C., Zhou, M., Ghosh, I. & Wirth, M. J., Feb 2007, In : Applied Spectroscopy. 61, 2, p. 130-137 8 p.

Research output: Contribution to journal › Article

delta Opioid Receptor

Peptides

peptides

Labels

Polarization

13 Citations (Scopus)

Profiling small molecule inhibitors against helix-receptor interactions: The Bcl-2 family inhibitor BH3I-1 potently inhibits p53/hDM2

Porter, J. R., Helmers, M. R., Wang, P., Furman, J. L., Joy, S. T., Arora, P. S. & Ghosh, I., Nov 14 2010, In : Chemical Communications. 46, 42, p. 8020-8022 3 p.

Research output: Contribution to journal › Article

Proteins

Molecules

11 Citations (Scopus)

Selection of cyclic-peptide inhibitors targeting Aurora kinase A: Problems and solutions

Shomin, C. D., Restituyo, E., Cox, K. J. & Ghosh, I., Nov 15 2011, In : Bioorganic and Medicinal Chemistry. 19, 22, p. 6743-6749 7 p.

Research output: Contribution to journal › Article

Aurora Kinases

Aurora Kinase A

Cyclic Peptides

Catalytic Domain

Adenosine Triphosphate

111 Citations (Scopus)

Selective amplification by auto- and cross-catalysis in a replicating peptide system

Yao, S., Ghosh, I., Zutshi, R. & Chmielewski, J., Dec 3 1998, In : Nature. 396, 6710, p. 447-450 4 p.

Research output: Contribution to journal › Article

Catalysis

Peptides

Oligonucleotides

Salts

45 Citations (Scopus)

Sequence-enabled reassembly of β-lactamase (SEER-LAC): A sensitive method for the detection of double-stranded DNA

Ooi, A. T., Stains, C. I., Ghosh, I. & Segal, D. J., Mar 21 2006, In : Biochemistry. 45, 11, p. 3620-3625 6 p.

Research output: Contribution to journal › Article

DNA

DNA sequences

Zinc Fingers

Zinc

Substitution reactions

9 Citations (Scopus)

Single-site mutations in a hyperthermophilic variant of the B1 domain of protein G result in self-assembled oligomers

Meyer, S. C., Huerta, C. & Ghosh, I., Feb 22 2005, In : Biochemistry. 44, 7, p. 2360-2368 9 p.

Research output: Contribution to journal › Article

Point Mutation

Oligomers

Congo Red

Mutation

Fluorescence Spectrometry

68 Citations (Scopus)

Site-specific detection of DNA methylation utilizing mCpG-SEER

Stains, C. I., Furman, J. L., Segal, D. J. & Ghosh, I., Aug 2 2006, In : Journal of the American Chemical Society. 128, 30, p. 9761-9765 5 p.

Research output: Contribution to journal › Article

DNA Methylation

Green Fluorescent Proteins

Proteins

Fluorescence

Zinc Fingers

9 Citations (Scopus)

Small molecule gated split-tyrosine phosphatases and orthogonal split-tyrosine kinases

Camacho-Soto, K., Castillo-Montoya, J., Tye, B., Ogunleye, L. O. & Ghosh, I., Dec 10 2014, In : Journal of the American Chemical Society. 136, 49, p. 17078-17086 9 p.

Research output: Contribution to journal › Article

Phosphatases

Sirolimus

Phosphoric Monoester Hydrolases

Protein-Tyrosine Kinases

Protein Kinases

88 Citations (Scopus)

Split-protein systems: Beyond binary protein-protein interactions

Shekhawat, S. S. & Ghosh, I., Dec 2011, In : Current Opinion in Chemical Biology. 15, 6, p. 790-797 8 p.

Research output: Contribution to journal › Article

Proteins

Ternary Complex Factors

Cataloging

Firefly Luciferases

Protein Engineering

44 Citations (Scopus)

Split β-lactamase sensor for the sequence-specific detection of DNA methylation

Porter, J. R., Stains, C. I., Segal, D. J. & Ghosh, I., Sep 1 2007, In : Analytical Chemistry. 79, 17, p. 6702-6708 7 p.

Research output: Contribution to journal › Article

Methylation

Genes

Sensors

Tumors

Green Fluorescent Proteins

20 Citations (Scopus)

Staurosporine tethered peptide ligands that target cAMP-dependent protein kinase (PKA): Optimization and selectivity profiling

Shomin, C. D., Meyer, S. C. & Ghosh, I., Sep 1 2009, In : Bioorganic and Medicinal Chemistry. 17, 17, p. 6196-6202 7 p.

Research output: Contribution to journal › Article

Staurosporine

Cyclic AMP-Dependent Protein Kinases

Phosphotransferases

Ligands

Peptides

5 Citations (Scopus)

Structure-function relationship in a β-sheet peptide Inhibitor of E47 dimerization and DNA binding

Ghosh, I., Issac, R. & Chmielewski, J., Jan 1999, In : Bioorganic and Medicinal Chemistry. 7, 1, p. 61-66 6 p.

Research output: Contribution to journal › Article

Dimerization

Transcription Factors

Alanine

Peptides

DNA

6 Citations (Scopus)

Synthesis of the basic-helix-loop-helix region of the immunoglobulin enhancer binding protein E47 and evaluation of its structural and DNA binding properties

Bishop, P., Jones, C., Ghosh, I. & Chmielewski, J., 1995, In : International Journal of Peptide and Protein Research. 46, 2, p. 149-154 6 p.

Research output: Contribution to journal › Article

NF-kappa B

Crystal structure

E-Box Elements

Solid-Phase Synthesis Techniques

Size exclusion chromatography

7 Citations (Scopus)

Systematic evaluation of split-fluorescent proteins for the direct detection of native and methylated DNA

Furman, J. L., Badran, A. H., Shen, S., Stains, C. I., Hannallah, J., Segal, D. J. & Ghosh, I., Jul 15 2009, In : Bioorganic and Medicinal Chemistry Letters. 19, 14, p. 3748-3751 4 p.

Research output: Contribution to journal › Article

Venus

Nucleic Acids

Nucleic acid sequences

Methylation

DNA

18 Citations (Scopus)

Testing the promiscuity of commercial kinase inhibitors against the AGC kinase group using a split-luciferase screen

Jester, B. W., Gaj, A., Shomin, C. D., Cox, K. J. & Ghosh, I., Feb 23 2012, In : Journal of Medicinal Chemistry. 55, 4, p. 1526-1537 12 p.

Research output: Contribution to journal › Article

Luciferases

Phosphotransferases

Catalytic Domain

Predictive Value of Tests

Competitive Binding