• 1885 Citations
  • 19 h-Index
1980 …2019
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Research Output 1992 2019

  • 1885 Citations
  • 19 h-Index
  • 41 Article
  • 1 Chapter
  • 1 Comment/debate
  • 1 Review article
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Article
2019
4 Citations (Scopus)

Endonuclease activity inhibition of the NS1 protein of parvovirus B19 as a novel target for antiviral drug development

Xu, P., Ganaie, S. S., Wang, X., Wang, Z., Kleiboeker, S., Horton, N. C., Heier, R. F., Meyers, M. J., Tavis, J. E. & Qiu, J., Mar 1 2019, In : Antimicrobial Agents and Chemotherapy. e01879-18.

Research output: Contribution to journalArticle

Endonucleases
Antiviral Agents
DNA Replication
Replication Origin
Terminal Repeat Sequences
2 Citations (Scopus)

Mechanism of Filamentation-Induced Allosteric Activation of the SgrAI Endonuclease

Polley, S., Lyumkis, D. & Horton, N. C., Oct 1 2019, In : Structure. 27, 10, p. 1497-1507.e3

Research output: Contribution to journalArticle

Open Access
Type II Site Specific Deoxyribonucleases
DNA Cleavage
DNA
Enzymes
endodeoxyribonuclease SgrAI
3 Citations (Scopus)

The Need for Speed: Run-On Oligomer Filament Formation Provides Maximum Speed with Maximum Sequestration of Activity

Barahona, C. J., Basantes, L. E., Tompkins, K. J., Heitman, D. M., Chukwu, B. I., Sanchez, J., Sanchez, J. L., Ghadirian, N., Park, C. K. & Horton, N. C., Mar 1 2019, In : Journal of virology. 93, 5

Research output: Contribution to journalArticle

DNA Cleavage
Enzymes
enzymes
DNA
Virus Diseases
2018
4 Citations (Scopus)

The run-on oligomer filament enzyme mechanism of SgrAI: Part 1. Assembly kinetics of the run-on oligomer filament

Park, C. K., Sanchez, J. L., Barahona, C., Basantes, L. E., Sanchez, J., Hernandez, C. & Horton, N. C., Jan 1 2018, In : Journal of Biological Chemistry. 293, 38, p. 14585-14598 14 p.

Research output: Contribution to journalArticle

DNA Cleavage
Oligomers
Kinetics
DNA
Enzymes
4 Citations (Scopus)

The run-on oligomer filament enzyme mechanism of SgrAI: Part 2. Kinetic modeling of the full DNA cleavage pathway

Park, C. K., Sanchez, J. L., Barahona, C., Basantes, L. E., Sanchez, J., Hernandez, C. & Horton, N. C., Jan 1 2018, In : Journal of Biological Chemistry. 293, 38, p. 14599-14615 17 p.

Research output: Contribution to journalArticle

DNA Cleavage
Oligomers
Kinetics
DNA
Type II Site Specific Deoxyribonucleases
2016
5 Citations (Scopus)

DNA Binding and Cleavage by the Human Parvovirus B19 NS1 Nuclease Domain

Sanchez, J. L., Romero, Z., Quinones, A., Torgeson, K. R. & Horton, N. C., Nov 29 2016, In : Biochemistry. 55, 47, p. 6577-6593 17 p.

Research output: Contribution to journalArticle

Erythema Infectiosum
Human Parvovirus B19
DNA Cleavage
Replication Origin
Transcriptional Activation
2015
7 Citations (Scopus)

Probing the run-on oligomer of activated SgrAI bound to DNA

Shah, S., Sanchez, J., Stewart, A., Piperakis, M. M., Cosstick, R., Nichols, C., Park, C. K., Ma, X., Wysocki, V., Bitinaite, J. & Horton, N. C., Apr 16 2015, In : PLoS One. 10, 4, e0124783.

Research output: Contribution to journalArticle

Oligomers
DNA
Chemical activation
Oligomerization
Type II Site Specific Deoxyribonucleases

Structure and specificity of FEN-1 from Methanopyrus kandleri

Shah, S., Dunten, P., Stiteler, A., Park, C. K. & Horton, N. C., 2015, In : Proteins: Structure, Function and Genetics. 83, 1, p. 188-194 7 p.

Research output: Contribution to journalArticle

Flap Endonucleases
DNA Repair
Repair
Nucleotides
Genomic Instability
2013
14 Citations (Scopus)

Allosteric regulation of DNA cleavage and sequence-specificity through run-on oligomerization

Lyumkis, D., Talley, H., Stewart, A., Shah, S., Park, C. K., Tama, F., Potter, C. S., Carragher, B. & Horton, N. C., Oct 8 2013, In : Structure. 21, 10, p. 1848-1858 11 p.

Research output: Contribution to journalArticle

Allosteric Regulation
DNA Cleavage
DNA
Proteins
Enzyme Activation
13 Citations (Scopus)

Structural analysis of activated SgrAI-DNA oligomers using ion mobility mass spectrometry

Ma, X., Shah, S., Zhou, M., Park, C. K., Wysocki, V. H. & Horton, N. C., Jun 25 2013, In : Biochemistry. 52, 25, p. 4373-4381 9 p.

Research output: Contribution to journalArticle

Oligomers
Structural analysis
Mass spectrometry
Mass Spectrometry
Ions
2011
8 Citations (Scopus)
DNA Cleavage
DNA
Type II Site Specific Deoxyribonucleases
Metals
Ions
2010
15 Citations (Scopus)

Activation of DNA cleavage by oligomerization of DNA-bound SgrAI

Park, C. K., Stiteler, A. P., Shah, S., Ghare, M. I., Bitinaite, J. & Horton, N. C., Oct 19 2010, In : Biochemistry. 49, 41, p. 8818-8830 13 p.

Research output: Contribution to journalArticle

Oligomerization
DNA Cleavage
Chemical activation
DNA
Molecular Weight
13 Citations (Scopus)

Domain swapping in allosteric modulation of DNA specificity

Park, C. K., Joshi, H. K., Agrawal, A., Ghare, M. I., Little, E. J., Dunten, P. W., Bitinaite, J. & Horton, N. C., Dec 2010, In : PLoS Biology. 8, 12, e1000554.

Research output: Contribution to journalArticle

Modulation
DNA
Dimers
DNA Cleavage
restriction endonucleases
2009

The restriction enzyme SgrAI: Structure solution via combination of poor MIRAS and MR phases

Dunten, P. W., Little, E. J. & Horton, N. C., Mar 19 2009, In : Acta Crystallographica Section D: Biological Crystallography. 65, 4, p. 393-398 6 p.

Research output: Contribution to journalArticle

Type II Site Specific Deoxyribonucleases
Electrons
Enzymes
DNA
Datasets
2008
7 Citations (Scopus)

DNA Distortion and Specificity in a Sequence-Specific Endonuclease

Babic, A. C., Little, E. J., Manohar, V. M., Bitinaite, J. & Horton, N. C., Oct 31 2008, In : Journal of Molecular Biology. 383, 1, p. 186-204 19 p.

Research output: Contribution to journalArticle

Endonucleases
DNA
Enzymes
Nucleic Acid Conformation
DNA Cleavage
16 Citations (Scopus)

Early Interrogation and Recognition of DNA Sequence by Indirect Readout

Little, E. J., Babic, A. C. & Horton, N. C., Dec 12 2008, In : Structure. 16, 12, p. 1828-1837 10 p.

Research output: Contribution to journalArticle

Recombinational DNA Repair
DNA
Enzymes
Proteins
GTYRAC-specific type II deoxyribonucleases
23 Citations (Scopus)

The structure of SgrAI bound to DNA; recognition of an 8 base pair target

Dunten, P. W., Little, E. J., Gregory, M. T., Manohar, V. M., Dalton, M., Hough, D., Bitinaite, J. & Horton, N. C., 2008, In : Nucleic Acids Research. 36, 16, p. 5405-5416 12 p.

Research output: Contribution to journalArticle

Base Pairing
DNA
Type II Site Specific Deoxyribonucleases
Divalent Cations
DNA-Binding Proteins
2006
21 Citations (Scopus)

Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism

Joshi, H. K., Etzkorn, C., Chatwell, L., Bitinaite, J. & Horton, N. C., Aug 18 2006, In : Journal of Biological Chemistry. 281, 33, p. 23852-23869 18 p.

Research output: Contribution to journalArticle

Type II Site Specific Deoxyribonucleases
DNA
Mutation
Base Pairing
DNA-Binding Proteins
74 Citations (Scopus)

Structure of Aart, a Designed Six-finger Zinc Finger Peptide, Bound to DNA

Segal, D. J., Crotty, J. W., Bhakta, M. S., Barbas, C. F. & Horton, N. C., Oct 20 2006, In : Journal of Molecular Biology. 363, 2, p. 405-421 17 p.

Research output: Contribution to journalArticle

Zinc Fingers
Fingers
Peptides
DNA
Proteins
2005
5 Citations (Scopus)
Zinc Fingers
Crystallization
Fingers
peptides
Zinc
3 Citations (Scopus)

DNA-induced conformational changes in type II restriction endonucleases: The structure of unliganded HincII

Little, E. J. & Horton, N. C., Aug 5 2005, In : Journal of Molecular Biology. 351, 1, p. 76-88 13 p.

Research output: Contribution to journalArticle

Type II Site Specific Deoxyribonucleases
DNA
Endonucleases
2004
24 Citations (Scopus)

Ca2+ binding in the active site of HincII: Implications for the catalytic mechanism

Etzkorn, C. & Horton, N. C., Oct 26 2004, In : Biochemistry. 43, 42, p. 13256-13270 15 p.

Research output: Contribution to journalArticle

Catalytic Domain
Type II Site Specific Deoxyribonucleases
Phosphates
Ions
DNA Cleavage
76 Citations (Scopus)

DNA cleavage by EcoRV endonuclease: Two metal ions in three metal ion binding sites

Horton, N. C. & Perona, J. J., Jun 8 2004, In : Biochemistry. 43, 22, p. 6841-6857 17 p.

Research output: Contribution to journalArticle

DNA Cleavage
Metal ions
Metals
Phosphates
Binding Sites
16 Citations (Scopus)

Mechanistic insights from the structures of HincII bound to cognate DNA cleaved from addition of Mg 2+ and Mn 2+

Etzkorn, C. & Horton, N. C., Oct 29 2004, In : Journal of Molecular Biology. 343, 4, p. 833-849 17 p.

Research output: Contribution to journalArticle

Catalytic Domain
Phosphates
DNA
Hydrogen Bonding
Ions
2002
21 Citations (Scopus)

Electrostatic contributions to site specific DNA cleavage by EcoRV endonuclease

Horton, N. C., Otey, C., Lusetti, S., Sam, M. D., Kohn, J., Martin, A. M., Ananthnarayan, V. & Perona, J. J., Sep 3 2002, In : Biochemistry. 41, 35, p. 10754-10763 10 p.

Research output: Contribution to journalArticle

DNA Cleavage
Static Electricity
Electrostatics
Catalytic Domain
DNA
48 Citations (Scopus)

Sequence selectivity and degeneracy of a restriction endonuclease mediated by DNA intercalation

Horton, N. C., Dorner, L. F. & Perona, J. J., 2002, In : Nature Structural Biology. 9, 1, p. 42-47 6 p.

Research output: Contribution to journalArticle

DNA Restriction Enzymes
Intercalation
DNA
Glutamine
Base Pairing
2001
17 Citations (Scopus)

Catalytic efficiency and sequence selectivity of a restriction endonuclease modulated by a distal manganese ion binding site

Sam, M. D., Horton, N. C., Nissan, T. A. & Perona, J. J., Mar 2 2001, In : Journal of Molecular Biology. 306, 4, p. 851-861 11 p.

Research output: Contribution to journalArticle

DNA Restriction Enzymes
Manganese
Binding Sites
Ions
DNA
43 Citations (Scopus)

Making the most of metal ions

Horton, N. C. & Perona, J. J., 2001, In : Nature Structural Biology. 8, 4, p. 290-293 4 p.

Research output: Contribution to journalArticle

Metal ions
Metals
Ions
Double-Stranded DNA Breaks
Deoxyribonuclease I
2000
42 Citations (Scopus)

Crystallographic snapshots along a protein-induced DNA-bending pathway

Horton, N. C. & Perona, J. J., May 23 2000, In : Proceedings of the National Academy of Sciences of the United States of America. 97, 11, p. 5729-5734 6 p.

Research output: Contribution to journalArticle

DNA
Proteins
Base Pairing
Catalytic Domain
Phosphates
33 Citations (Scopus)

Inhibition of EcoRV endonuclease by deoxyribo-3'-S-phosphorothiolates: A high-resolution X-ray crystallographic study

Horton, N. C., Connolly, B. A. & Perona, J. J., Apr 12 2000, In : Journal of the American Chemical Society. 122, 14, p. 3314-3324 11 p.

Research output: Contribution to journalArticle

Metal ions
DNA
Metals
Phosphates
X-Rays
1999
7 Citations (Scopus)

Crystallization and preliminary diffraction analysis of the HincII restriction endonuclease-DNA complex

Horton, N. C., Dorner, L. F., Schildkraut, I. & Perona, J. J., Nov 1999, In : Acta Crystallographica Section D: Biological Crystallography. 55, 11, p. 1943-1945 3 p.

Research output: Contribution to journalArticle

Synchrotrons
DNA Restriction Enzymes
Crystallization
Base Pairing
constrictions
61 Citations (Scopus)

Divalent metal dependence of site-specific DNA binding by EcoRV endonuclease

Martin, A. M., Horton, N. C., Lusetti, S., Reich, N. O. & Perona, J. J., Jun 29 1999, In : Biochemistry. 38, 26, p. 8430-8439 10 p.

Research output: Contribution to journalArticle

Metals
Metal ions
DNA
Ions
DNA Restriction Enzymes
1998
61 Citations (Scopus)

Cation binding to the integrin CD11b I domain and activation model assessment

Baldwin, E. T., Sarver, R. W., Bryant, G. L., Curry, K. A., Fairbanks, M. B., Finzel, B. C., Garlick, R. L., Heinrikson, R. L., Horton, N. C., Kelley, L. L. C., Mildner, A. M., Moon, J. B., Mott, J. E., Mutchler, V. T., Tomich, C. S. C., Watenpaugh, K. D. & Wiley, V. H., Jul 15 1998, In : Structure. 6, 7, p. 923-935 13 p.

Research output: Contribution to journalArticle

Integrins
Cations
Ions
Metals
Cell Surface Receptors
100 Citations (Scopus)

Metal ion-mediated substrate-assisted catalysis in type II restriction endonucleases

Horton, N. C., Newberry, K. J. & Perona, J. J., Nov 10 1998, In : Proceedings of the National Academy of Sciences of the United States of America. 95, 23, p. 13489-13494 6 p.

Research output: Contribution to journalArticle

Type II Site Specific Deoxyribonucleases
Catalysis
Metals
Phosphates
Ions
32 Citations (Scopus)

Recognition of flanking DNA sequences by EcoRV endonuclease involves alternative patterns of water-mediated contacts

Horton, N. C. & Perona, J. J., Aug 21 1998, In : Journal of Biological Chemistry. 273, 34, p. 21721-21729 9 p.

Research output: Contribution to journalArticle

DNA sequences
Water
Molecules
Thymine
Cytosine
37 Citations (Scopus)
DNA
Proteins
Base Pairing
Phosphates
Costs and Cost Analysis
1997
19 Citations (Scopus)

Escherichia coli lac repressor-lac operator interaction and the influence of allosteric effectors

Horton, N. C., Lewis, M. & Lu, P., Jan 10 1997, In : Journal of Molecular Biology. 265, 1, p. 1-7 7 p.

Research output: Contribution to journalArticle

Lac Repressors
Escherichia coli
Base Pairing
DNA
Isopropyl Thiogalactoside
1996
615 Citations (Scopus)

Crystal structure of the lactose operon represser and its complexes with DNA and inducer

Lewis, M., Chang, G., Horton, N. C., Kercher, M. A., Pace, H. C., Schumacher, M. A., Brennan, R. G. & Lu, P., Mar 1 1996, In : Science. 271, 5253, p. 1247-1254 8 p.

Research output: Contribution to journalArticle

Lac Repressors
Lac Operon
DNA
Base Pairing
Cyclic AMP Receptor Protein

Structure and genetics of the lactose operon repressor

Kercher, M. A., Chang, G., Horton, N. C., Lu, P., Miller, J. H., Pace, H. C. & Lewis, M., 1996, In : FASEB Journal. 10, 6

Research output: Contribution to journalArticle

Lac Repressors
Lac Operon
Genetic Structures
operon
Lactose
77 Citations (Scopus)

The structure of an RNA/DNA hybrid: A substrate of the ribonuclease activity of HIV-1 reverse transcriptase

Horton, N. C. & Finzel, B. C., Dec 6 1996, In : Journal of Molecular Biology. 264, 3, p. 521-533 13 p.

Research output: Contribution to journalArticle

Ribonucleases
RNA
DNA
Base Pairing
Deoxyribonucleotides
1992
311 Citations (Scopus)

Calculation of the free energy of association for protein complexes

Horton, N. C. & Lewis, M., 1992, In : Protein Science. 1, 1, p. 169-181 13 p.

Research output: Contribution to journalArticle

Free Association
Nuclear Energy
Virus Assembly
Entropy
Free energy