ANALYSIS OF A NATURAL KILLER CELL TARGET CELL ANTIGEN

Project: Research project

Description

Cellular immunity mediated by nonspecific cytotoxic cells (NCC)
in catfish and natural killer cells (NK) in mice and humans will be
analyzed and compared. This unique approach will be
accomplished with monoclonal antibodies (MAbs) which have been
derived against target cell antigens involved in the recognition
function of NCC and NK. These MAbs inhibit the lysis of target
cells by both NCC and NK. Using these MAbs as biological probes
studies will be performed to: (a) define the species distribution
and expression of the target cell molecules; (b) examine the
biological role of these structures in immune function; and (c)
determine the similarity of these molecules to other known
antigens. At the biochemical level, analyses will be made of the
primary structure of these molecules (e.g., glycosylation). Studies
at the molecular level will examine the regulation of these
molecules and attempt to identify and isolate the gene(s) encoding
these molecules. Studies proposed in this grant will contribute significant
knowledge to the field of comparative immunology. More
importantly, the studies will address a major question in NK cell
biology: What molecules on the surface of target cells are
recognized by NK cells? And what role do these molecules serve
in normal immune function? To achieve these aims, the highly sensitive technique of flow
cytometry in combination with various biochemical and molecular
analyses will be utilized to study those molecules in fish, mice and
humans. The ultimate goal of the project is to fully characterize
a novel structure involved in immune function across species
barriers.
StatusFinished
Effective start/end date7/15/886/30/94

Funding

  • National Institutes of Health: $150,930.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $148,091.00
  • National Institutes of Health
  • National Institutes of Health

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Natural Killer Cells
Antigens
Monoclonal Antibodies
Catfishes
Organized Financing
Allergy and Immunology
Glycosylation
Cellular Immunity
Fishes
Genes

ASJC

  • Medicine(all)