Genetics of Host Resistance to Franciscella tularensis in Mice

Project: Research project

Description

DESCRIPTION (provided by applicant): Understanding the genetics of host resistance and susceptibility to Franciscella tularensis can provide important clues into the interaction between pathogens and their hosts. They can reveal critical molecules involved in both pathogenesis e.g. receptors used by pathogens, and host defense mechanisms to which the pathogen is exquisitely sensitive. It can uncover important components of both the innate and acquired immune system. We will perform a Genome Scan of F2 populations derived from resistant C57Bl/6J and susceptible A/J, and resistant BALB/cJ and susceptible A/J. To confirm QTL linkage we will perform backcross selection using FT resistance for selection. We will perform a backcross to the susceptible parent using F2 progeny that have the lowest bacterial load. This allows an enrichment of the relevant genes at the same time as recombination allows closer localization. This project will identify genes, and pathways that are critical in the ability of hosts to fight off bacterial infections. It concentrates on the resistance to the bacteria that causes rabbit fever, Franciscella tularensis. Understanding the genes involved will lead to better treatments, and better vaccines.
StatusFinished
Effective start/end date8/15/077/31/10

Funding

  • National Institutes of Health: $219,000.00
  • National Institutes of Health: $179,033.00

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mice
pathogens
genes
bacterial infections
defense mechanisms
fever
linkage (genetics)
quantitative trait loci
pathogenesis
concentrates
rabbits
vaccines
receptors
genome
bacteria
innate immunity
adaptive immunity

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)