MOLECULAR GENETIC ANALYSIS OF T. CRUZI NEURAMINIDASE

Project: Research project

Description

Many protozoa which are members of the Kinetoplastida family have
complicated life cycle involving several stages of morphogenesis. To date
little is known about the genetic regulation of expression of stage
specific genes in these microorganisms or the contribution each stage
specific surface protein makes to the biology of the organism (with the
exception of the VSG genes of Trypanosoma brucei). The neuraminidase is a developmentally regulated enzyme in Trypanosoma
cruzi and a possible virulence factor. We will use a combination of
recombinant DNA technology, protein biochemistry, and immunological
techniques to characterize the enzyme and to study the regulatory mechanism
governing the expression of the gene. Our experiments will serve as a
model for future studies aimed at identifying the components important for
intracellular survival of this organism.
StatusFinished
Effective start/end date1/1/8612/31/92

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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sialidase
Kinetoplastida
Salmonella
molecular genetics
genetic techniques and protocols
Trypanosoma brucei
family relations
organisms
surface proteins
gene expression regulation
enzymes
Antigens
biochemistry
morphogenesis
Protozoa
life cycle (organisms)
virulence
Genes
microorganisms
Biological Sciences

ASJC

  • Medicine(all)
  • Immunology and Microbiology(all)