α2-Adrenergic receptors stimulate cell migration and decrease cytoskeletal organization in rat aortic smooth muscle cells

John W Regan, J. G. Richman

Research output: Contribution to journalArticle

Abstract

Vascular wound healing and pathology is characterized by migration and proliferation of medial smooth muscle cells following denudation of the intima. To explore potential physiological roles that α2-adrenergic receptors (α2-ARs) may play in smooth muscle, the presence of the three subtypes as well as the functions they mediate, were examined. Immunofluorescent microscopy, revealed the presence of all three subtypes in explant-derived cultures of rat aortic smooth muscle (RASM) cells. The expression of all three subtypes was verified using RT-PCR. Stimulation of α2-ARs with the selective agonist dexmedetomidine (Dex) lead to a 5-fold increase in MAP kinase activity. This was blocked in the presence of the antagonist rauwolscine (RW) as well as by treatment with pertussis toxin. Interestingly, treatment of RASM cells with Dex stimulated cell migration which could be blocked by co-incubation with RW. Dex, however, did not stimulate cell proliferation as measured by [3H]thymidine incorporation. Incubation of RASM cells with Dex also produced a marked decrease in f-actin labeling, which again, was prevented by co-incubation with RW. The evidence reveals the presence of functional α2-ARs in RASM cells. The involvement of α2-ARs with cell migration and cytoskeletal changes is novel and indicates a potential role in vascular wound healing or pathogenesis.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

adrenergic receptors
Dexmedetomidine
cell movement
dexmedetomidine
smooth muscle
myocytes
Adrenergic Receptors
Smooth Muscle Myocytes
Cell Movement
Muscle
Rats
Cells
Yohimbine
rats
Wound Healing
Blood Vessels
tissue repair
blood vessels
Pertussis Toxin
pertussis toxin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

α2-Adrenergic receptors stimulate cell migration and decrease cytoskeletal organization in rat aortic smooth muscle cells. / Regan, John W; Richman, J. G.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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