β-Adrenergic receptors regulate astrogliosis and cell proliferation in the central nervous system in vivo

Cheryl Hodges-Savola, Scott D. Rogers, Joseph R. Ghilardi, David R. Timm, Patrick W Mantyh

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Astrocytes express several cell surface receptors including the β2- adrenergic receptor. To explore whether β-adrenergic receptors (β-ARs) directly regulate astrogliosis and glial scar formation, we evaluated the effects of β-AR activation and blockade on astrocyte hypertrophy and cell proliferation in rabbit optic nerves in vivo. Artificial cerebrospinal fluid (CSF), isoproterenol (ISO; a β-agonist), or propranolol (PROP; a β- antagonist) were infused via osmotic minipumps into non-injured and crushed optic nerves for 14 days. Changes in nerve cell numbers and astroglial hypertrophy were monitored by ethidium bromide nuclear staining and glial fibrillary acidic protein (GFAP) immunohistochemistry, respectively. In non- injured nerves infused with CSF or PROP, there were no alterations in GFAP- immunoreactivity or cell numbers compared to normal optic nerves; however, in non-injured nerves infused with ISO, there was a significant increase in both GFAP-immunoreactivity and cell number. In crushed optic nerves, there was a significant increase in both GFAP-immunoreactivity and cell number compared to normal nerves, and this increase was not altered by infusion of either CSF or ISO. In contrast, PROP infusion significantly reduced the crush-induced increase in GFAP-immunofluorescence and cell number. These findings suggest that a) β-AR activation, in the absence of injury, can promote astroglial hypertrophy and cell proliferation; b) after injury, β- AR activation drives injury-induced astrogliosis and cell proliferation; c) astrocyte β-ARs are maximally stimulated after neuronal injury; and d) neuronal regeneration may be influenced, both positively and negatively, through the pharmacological manipulation of glial receptors.

Original languageEnglish (US)
Pages (from-to)52-62
Number of pages11
JournalGLIA
Volume17
Issue number1
DOIs
StatePublished - May 1996
Externally publishedYes

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Glial Fibrillary Acidic Protein
Adrenergic Receptors
Optic Nerve
Central Nervous System
Cell Count
Cell Proliferation
Astrocytes
Hypertrophy
Cerebrospinal Fluid
Wounds and Injuries
Neuroglia
Ethidium
Cell Surface Receptors
Isoproterenol
Propranolol
Cicatrix
Fluorescent Antibody Technique
Regeneration
Immunohistochemistry
Pharmacology

Keywords

  • Astroglia
  • Glia
  • Noradrenaline
  • Regeneration

ASJC Scopus subject areas

  • Immunology

Cite this

β-Adrenergic receptors regulate astrogliosis and cell proliferation in the central nervous system in vivo. / Hodges-Savola, Cheryl; Rogers, Scott D.; Ghilardi, Joseph R.; Timm, David R.; Mantyh, Patrick W.

In: GLIA, Vol. 17, No. 1, 05.1996, p. 52-62.

Research output: Contribution to journalArticle

Hodges-Savola, Cheryl ; Rogers, Scott D. ; Ghilardi, Joseph R. ; Timm, David R. ; Mantyh, Patrick W. / β-Adrenergic receptors regulate astrogliosis and cell proliferation in the central nervous system in vivo. In: GLIA. 1996 ; Vol. 17, No. 1. pp. 52-62.
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