β2-Adrenoceptors regulate induction of myocardial ornithine decarboxylase in mice in vivo

J. G. Copeland, Douglas F Larson, William R Roeske, D. H. Russell, J. R. Womble

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Abstract

The pharmacological characteristics of the myocardial adrenoceptor of the mouse have been examined during embryogenesis by measuring ornithine decarboxylase (ODC, EC 4.1.1.17) induction. A four fold elevation of ODC activity was observed after isoprenaline (10 mg/kg, s.c.), and enzyme activity was increased two to three fold following adrenaline (1 mg/kg, s.c.) or terbutaline given by direct injection to the foetus (10 μg/500 mg). Pretreatment with the β-adrenoceptor antagonist, propranolol (10 mg/kg), totally blocked the increase in ODC activity. Elevation of myocardial ODC activity was not inhibited by metoprolol, a relatively specific β1-adrenoceptor antagonist, at a dose of 10 mg/kg. Since the increase in ODC activity was blocked by a β-adrenoceptor antagonist (propranolol) and enzyme activity was stimulated by terbutaline, a β2-agonist, we conclude that β2-adrenoceptors are selectively coupled to the regulation of murine cardiac ODC activity following catecholamine stimulation.

Original languageEnglish (US)
Pages (from-to)479-483
Number of pages5
JournalBritish Journal of Pharmacology
Volume75
Issue number3
StatePublished - 1982

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Ornithine Decarboxylase
Adrenergic Receptors
Terbutaline
Propranolol
Metoprolol
Enzymes
Isoproterenol
Epinephrine
Catecholamines
Embryonic Development
Fetus
Pharmacology
Injections

ASJC Scopus subject areas

  • Pharmacology

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β2-Adrenoceptors regulate induction of myocardial ornithine decarboxylase in mice in vivo. / Copeland, J. G.; Larson, Douglas F; Roeske, William R; Russell, D. H.; Womble, J. R.

In: British Journal of Pharmacology, Vol. 75, No. 3, 1982, p. 479-483.

Research output: Contribution to journalArticle

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