Δcps1 vaccine protects dogs against experimentally induced coccidioidomycosis

Lisa F. Shubitz, Edward J. Robb, Daniel A. Powell, Richard A. Bowen, Angela Bosco-Lauth, Airn Hartwig, Stephanie M. Porter, Hien Trinh, Hilary Moale, Helle Bielefeldt-Ohmann, James Hoskinson, Marc J. Orbach, Jeffrey A. Frelinger, John N. Galgiani

Research output: Contribution to journalArticlepeer-review

Abstract

Coccidioidomycosis is a significant health problem of dogs and humans in endemic regions, especially California and Arizona in the U.S. Both species would greatly benefit from a vaccine to prevent this disease. A live avirulent vaccine candidate, Δcps1, was tested for tolerability and efficacy to prevent pulmonary coccidioidomycosis in a canine challenge model. Vaccine injection-site reactions were transient and there were no systemic effects observed. Six of seven vaccine sites tested and all draining lymph nodes were sterile post-vaccination. Following infection with Coccidioides posadasii, strain Silveira, arthroconidia into the lungs, dogs given primary and booster vaccinations had significantly reduced lung fungal burdens (P = 0.0003) and composite disease scores (P = 0.0002) compared to unvaccinated dogs. Dogs vaccinated once had fungal burdens intermediate between those given two doses or none, but disease scores were not significantly different from unvaccinated (P = 0.675). Δcps1 was well-tolerated in the dogs and it afforded a high level of protection when given as prime and boost. These results drive the Δcps1 vaccine toward a licensed veterinary vaccine and support continued development of this vaccine to prevent coccidioidomycosis in humans.

Original languageEnglish (US)
Pages (from-to)6894-6901
Number of pages8
JournalVaccine
Volume39
Issue number47
DOIs
StatePublished - Nov 16 2021

Keywords

  • Avirulent
  • Coccidioidomycosis
  • Dogs
  • Fungal
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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