1α hydroxyvitamin D3: a synthetic sterol which is highly active in preventing rickets in the chick

D. J. Cork, Mark R Haussler, M. J. Pitt

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The antirachitic effectiveness of 1α hydroxyvitamin D3 (1α OH D3) was investigated by raising chicks on a vitamin D deficient diet and chronically administering various levels of this synthetic sterol. Vitamin D3 (D3) and 1α,25 dihydroxyvitamin D3 (1α,25 (OH)2 D3) were concurrently tested as dietary supplements in order to compare the potency of these three sterols. Growth, plasma calcium and phosphorus concentration, percentage bone ash, calcium absorption, and radiographic appearance of bone were determined after treatment of the chicks with the sterols for 3 weeks. 1α OH D3 was 5.1, 3.4 and 3.7 times more potent than D3 in stimulating weight gain, maintenance of plasma calcium and promotion of increased percent bone ash, respectively. Radiographic examination of tibiae and femurs from the various groups revealed that 1α OH D3 effectively mediates normal bone calcification; 1α OH D3 was 2-6 times more potent than D3 in this regard. The synthetic 1α OH D3 was slightly more active than 1α,25 (OH)2 D3 in most of the parameters tested, but appeared similar to 1α,25 (OH)2 D3 in terms of preventing radiologic evidence of rickets. 1α OH D3 was an efficient mediator of intestinal calcium absorption and exhibited a more sustained action than 1α,25 (OH)2 D3. Curative levels of all three sterols produced comparable suppression of renal 25 hydroxyvitamin D3 1α hydroxylase, an enzyme which has been previously shown to be regulated by vitamin D and calcium status. In conclusion, these data establish that 1α OH D3 is a potent analog of D3 which is capable of mimicking the natural hormone 1α,25 (OH)2 D3. The finding that 1α OH D3 produces normal bone development at extremely low concentrations suggests that this analog may be a useful therapeutic agent in endocrine related metabolic bone diseases.

Original languageEnglish (US)
Pages (from-to)1337-1345
Number of pages9
JournalEndocrinology
Volume94
Issue number5
StatePublished - 1974

Fingerprint

Rickets
Sterols
Calcium
Vitamin D
Calcifediol
Bone and Bones
Calcitriol
Metabolic Bone Diseases
Cholecalciferol
Bone Development
Intestinal Absorption
Dietary Supplements
Mixed Function Oxygenases
Tibia
Femur
Phosphorus
Weight Gain
Maintenance
Hormones
Diet

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

1α hydroxyvitamin D3 : a synthetic sterol which is highly active in preventing rickets in the chick. / Cork, D. J.; Haussler, Mark R; Pitt, M. J.

In: Endocrinology, Vol. 94, No. 5, 1974, p. 1337-1345.

Research output: Contribution to journalArticle

@article{93bc3d3c046f4d28b8a0371a57b874ee,
title = "1α hydroxyvitamin D3: a synthetic sterol which is highly active in preventing rickets in the chick",
abstract = "The antirachitic effectiveness of 1α hydroxyvitamin D3 (1α OH D3) was investigated by raising chicks on a vitamin D deficient diet and chronically administering various levels of this synthetic sterol. Vitamin D3 (D3) and 1α,25 dihydroxyvitamin D3 (1α,25 (OH)2 D3) were concurrently tested as dietary supplements in order to compare the potency of these three sterols. Growth, plasma calcium and phosphorus concentration, percentage bone ash, calcium absorption, and radiographic appearance of bone were determined after treatment of the chicks with the sterols for 3 weeks. 1α OH D3 was 5.1, 3.4 and 3.7 times more potent than D3 in stimulating weight gain, maintenance of plasma calcium and promotion of increased percent bone ash, respectively. Radiographic examination of tibiae and femurs from the various groups revealed that 1α OH D3 effectively mediates normal bone calcification; 1α OH D3 was 2-6 times more potent than D3 in this regard. The synthetic 1α OH D3 was slightly more active than 1α,25 (OH)2 D3 in most of the parameters tested, but appeared similar to 1α,25 (OH)2 D3 in terms of preventing radiologic evidence of rickets. 1α OH D3 was an efficient mediator of intestinal calcium absorption and exhibited a more sustained action than 1α,25 (OH)2 D3. Curative levels of all three sterols produced comparable suppression of renal 25 hydroxyvitamin D3 1α hydroxylase, an enzyme which has been previously shown to be regulated by vitamin D and calcium status. In conclusion, these data establish that 1α OH D3 is a potent analog of D3 which is capable of mimicking the natural hormone 1α,25 (OH)2 D3. The finding that 1α OH D3 produces normal bone development at extremely low concentrations suggests that this analog may be a useful therapeutic agent in endocrine related metabolic bone diseases.",
author = "Cork, {D. J.} and Haussler, {Mark R} and Pitt, {M. J.}",
year = "1974",
language = "English (US)",
volume = "94",
pages = "1337--1345",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "5",

}

TY - JOUR

T1 - 1α hydroxyvitamin D3

T2 - a synthetic sterol which is highly active in preventing rickets in the chick

AU - Cork, D. J.

AU - Haussler, Mark R

AU - Pitt, M. J.

PY - 1974

Y1 - 1974

N2 - The antirachitic effectiveness of 1α hydroxyvitamin D3 (1α OH D3) was investigated by raising chicks on a vitamin D deficient diet and chronically administering various levels of this synthetic sterol. Vitamin D3 (D3) and 1α,25 dihydroxyvitamin D3 (1α,25 (OH)2 D3) were concurrently tested as dietary supplements in order to compare the potency of these three sterols. Growth, plasma calcium and phosphorus concentration, percentage bone ash, calcium absorption, and radiographic appearance of bone were determined after treatment of the chicks with the sterols for 3 weeks. 1α OH D3 was 5.1, 3.4 and 3.7 times more potent than D3 in stimulating weight gain, maintenance of plasma calcium and promotion of increased percent bone ash, respectively. Radiographic examination of tibiae and femurs from the various groups revealed that 1α OH D3 effectively mediates normal bone calcification; 1α OH D3 was 2-6 times more potent than D3 in this regard. The synthetic 1α OH D3 was slightly more active than 1α,25 (OH)2 D3 in most of the parameters tested, but appeared similar to 1α,25 (OH)2 D3 in terms of preventing radiologic evidence of rickets. 1α OH D3 was an efficient mediator of intestinal calcium absorption and exhibited a more sustained action than 1α,25 (OH)2 D3. Curative levels of all three sterols produced comparable suppression of renal 25 hydroxyvitamin D3 1α hydroxylase, an enzyme which has been previously shown to be regulated by vitamin D and calcium status. In conclusion, these data establish that 1α OH D3 is a potent analog of D3 which is capable of mimicking the natural hormone 1α,25 (OH)2 D3. The finding that 1α OH D3 produces normal bone development at extremely low concentrations suggests that this analog may be a useful therapeutic agent in endocrine related metabolic bone diseases.

AB - The antirachitic effectiveness of 1α hydroxyvitamin D3 (1α OH D3) was investigated by raising chicks on a vitamin D deficient diet and chronically administering various levels of this synthetic sterol. Vitamin D3 (D3) and 1α,25 dihydroxyvitamin D3 (1α,25 (OH)2 D3) were concurrently tested as dietary supplements in order to compare the potency of these three sterols. Growth, plasma calcium and phosphorus concentration, percentage bone ash, calcium absorption, and radiographic appearance of bone were determined after treatment of the chicks with the sterols for 3 weeks. 1α OH D3 was 5.1, 3.4 and 3.7 times more potent than D3 in stimulating weight gain, maintenance of plasma calcium and promotion of increased percent bone ash, respectively. Radiographic examination of tibiae and femurs from the various groups revealed that 1α OH D3 effectively mediates normal bone calcification; 1α OH D3 was 2-6 times more potent than D3 in this regard. The synthetic 1α OH D3 was slightly more active than 1α,25 (OH)2 D3 in most of the parameters tested, but appeared similar to 1α,25 (OH)2 D3 in terms of preventing radiologic evidence of rickets. 1α OH D3 was an efficient mediator of intestinal calcium absorption and exhibited a more sustained action than 1α,25 (OH)2 D3. Curative levels of all three sterols produced comparable suppression of renal 25 hydroxyvitamin D3 1α hydroxylase, an enzyme which has been previously shown to be regulated by vitamin D and calcium status. In conclusion, these data establish that 1α OH D3 is a potent analog of D3 which is capable of mimicking the natural hormone 1α,25 (OH)2 D3. The finding that 1α OH D3 produces normal bone development at extremely low concentrations suggests that this analog may be a useful therapeutic agent in endocrine related metabolic bone diseases.

UR - http://www.scopus.com/inward/record.url?scp=0016138683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016138683&partnerID=8YFLogxK

M3 - Article

C2 - 4362967

AN - SCOPUS:0016138683

VL - 94

SP - 1337

EP - 1345

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 5

ER -