1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome

Yurong Guo, Shwu Fan Ma, Dmitry Grigoryev, Jennifer Van Eyk, Joe G.N. Garcia

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Bronchoalveolar lavage fluid (BALF) is a complex mixture of proteins, which represents a unique clinically useful sampling of the lower respiratory tract. Many proteomic technologies can be used to characterize complex biological mixtures; however, it is not yet clear which technology(s) provide more information regarding the number of proteins identified and sequence coverage. In this study, we initially compared two common proteomic approaches, 2-D LC microESI MS/ MS and 1-DE followed by gel slice digestion, peptide extraction and peptide identification by MS in characterization of the mouse BALF proteome; secondly, we identified 297 unique proteins from the mouse BALF proteome, greatly expanded the BALF proteome by about threefold regardless of species.

Original languageEnglish (US)
Pages (from-to)4608-4624
Number of pages17
JournalProteomics
Volume5
Issue number17
DOIs
StatePublished - Nov 2005
Externally publishedYes

Keywords

  • 1-DE
  • Bronchoalveolar lavage
  • LC-MS/MS
  • Mouse
  • Proteome analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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