1,25-Dihydroxyvitamin D3-glycoside: Identification of a calcinogenic principle of solanum malacoxylon

Mark R. Haussler, Robert H. Wasserman, Toni A. McCain, Meinrad Peterlik, Kristine M. Bursac, Mark R. Hughes

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

The water soluble calcinogenic factor present in the plant Solanum malacoxylon is partially purified by selective extraction and chromatography on silicic acid and then hydrolyzed with a mixed preparation of glycosidases from the sea worm, Charonia lampus. Hydrolysis produces a chloroform soluble factor with biologic characteristics of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the hormonal form of vitamin D. Purification of this factor is accomplished by chromatography on Sephadex LH-20, silicic acid, and Celite columns, yielding 3 μg of active material. During the isolation, bioactivity (as assessed by the ability of fractions to compete with labeled 1,25-(OH)2D3 for binding to a specific intestinal receptor protein) migrates exactly with authentic tritiated 1,25-(OH)2D3. The purified factor has an ultraviolet absorption spectrum identical to that of 1,25-(OH)2D3 and analysis via direct probe mass spectrometry yields a parent molecular ion of m/e 416 and a fragmentation pattern indistinguishable from synthetic 1,25-(OH)2D3 hormone. We therefore conclude that the vitamin D-like principle in Solanum malacoxylon is a sterol-glycoside which contains the 1,25-(OH)2D3 molecule as its active sterol component.

Original languageEnglish (US)
Pages (from-to)1049-1056
Number of pages8
JournalLife Sciences
Volume18
Issue number10
DOIs
StatePublished - May 15 1976

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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