1,25-Dihydroxyvitamin D3 receptors in rat kidney cytosol

J. S. Chandler, J. W. Pike, M. R. Haussler

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Abstract

Rat kidney cytosol contains a 3.3 S high affinity binding component for 1,25-dihydroxyvitamin D3 as detected by DNA-cellulose chromatography and subsequent sucrose gradient analysis. The semipurified aporeceptor demonstrates specificity for 1,25-dihydroxyvitamin D3 and an apparent dissociation constant for this sterol-hormone of 3.4 × 10-10M at 25°C. The physicochemical properties of this binding component are in agreement with those observed for the chick intestinal 1,25-dihydroxyvitamin D3 receptor, suggesting that this component may function as a specific receptor for the hormone in the kidney.

Original languageEnglish (US)
Pages (from-to)1057-1063
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume90
Issue number3
DOIs
StatePublished - Oct 12 1979

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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