1H N.M.R. STUDY OF THE CONFORMATION OF [Glu 4] OXYTOCIN AND ITS LANTHANIDE COMPLEXES IN AQUEOUS SOLUTION

R. WALTER, C. W. SMITH, K. P. SARATHY, R. P. PILLAI, N. R. KRISHNA, R. E. LENKINSKI, J. D. GLICKSON, Victor J Hruby

Research output: Contribution to journalArticle

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Abstract

At 400 MHz the 1H chemical shifts, peptide NH‐CαH coupling constants (J) and peptide hydrogen exchange rates of [Glu4] oxytocin in aqueous solution closely resemble those previously reported for oxytocin under comparable conditions, indicating that both the parent hormone and its analogue have similar conformations in this solvent. The hydrogen exchange data suggest a dynamic equilibrium between conformation(s) in which the peptide NH's of Asn5 Cys6 are internally hydrogen bonded and conformation(s) in which these hydrogens are bonded to the solvent. [Glu4]oxytocin forms 1:1 complexes with lanthanide metal ions. The diamagnetic La3+ complex exhibits values of J very similar to those of the metal free hormone analogue, suggesting that coordination of the metal is accompanied by minimal perturbation of the peptide backbone. Specific average proton‐metal distances estimated from Gd3+ induced paramagnetic relaxation effects indicate that the metal is probably coordinated to the Glu4 carboxyl group and the sidechain carbonyl of Asn5. Limiting shifts induced by binding of paramagnetic Yb3+ are also reported.

Original languageEnglish (US)
Pages (from-to)56-64
Number of pages9
JournalInternational Journal of Peptide and Protein Research
Volume17
Issue number1
DOIs
StatePublished - 1981

Fingerprint

Lanthanoid Series Elements
Conformations
Hydrogen
Oxytocin
Metals
Nuclear magnetic resonance
Peptides
Hormones
Chemical shift
Electronic data interchange
Metal ions
Ions
Glu(4)-oxytocin
Proton Magnetic Resonance Spectroscopy

Keywords

  • hydrogen exchange kinetics
  • lanthanamide metal ions
  • metal complexes
  • nuclear magnetic resonance
  • paramagnetic shift and relaxation probes
  • peptide hormone
  • solution conformation

ASJC Scopus subject areas

  • Biochemistry

Cite this

1H N.M.R. STUDY OF THE CONFORMATION OF [Glu 4] OXYTOCIN AND ITS LANTHANIDE COMPLEXES IN AQUEOUS SOLUTION. / WALTER, R.; SMITH, C. W.; SARATHY, K. P.; PILLAI, R. P.; KRISHNA, N. R.; LENKINSKI, R. E.; GLICKSON, J. D.; Hruby, Victor J.

In: International Journal of Peptide and Protein Research, Vol. 17, No. 1, 1981, p. 56-64.

Research output: Contribution to journalArticle

WALTER, R. ; SMITH, C. W. ; SARATHY, K. P. ; PILLAI, R. P. ; KRISHNA, N. R. ; LENKINSKI, R. E. ; GLICKSON, J. D. ; Hruby, Victor J. / 1H N.M.R. STUDY OF THE CONFORMATION OF [Glu 4] OXYTOCIN AND ITS LANTHANIDE COMPLEXES IN AQUEOUS SOLUTION. In: International Journal of Peptide and Protein Research. 1981 ; Vol. 17, No. 1. pp. 56-64.
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