3D image reconstruction using optical sectioning in confocal scanning microscopy

Jung Woo Seo, Dong Kyun Kang, Sung Lim Park, Dae Gab Gweon

Research output: Contribution to journalConference article

Abstract

Confocal scanning microscopy (CSM) has been used in biological application, materials science, semiconductor quality measurement and other non-destructive microscopic application. Small spot of light illuminates a sample, and a small detector that is ideally a point detector collects the reflected or transmitted light having the information of specimen. An image distribution can be reconstructed by a correlation analysis of spots with the high bandwidth. The mechanism for two-dimensional beam scanning and optical sectioning has an important role in CSM as the three-dimensional profiler. The parasitic motion of focus on the detector gives rise to the fatal distortion of an image profile named the extinction effect while using acousto-optical (AO) deflector. We propose the progressive methods for the high quality image as the following. At first, for having the corrected image, small spot and long scan range, this paper shows that the optimal design having the multi-objects can be used by choosing the unitary lens device in CSM. At second, in order to compensate for the intensity cancellation at the end profile that may be the cause of waviness for the optical image, this paper shows that it is efficient to schedule the frequency of scan. According to characteristics of the extinction curve and axial/lateral response having the error property, we can define the frequency and sensitivity of as their robustness. Finally, the axial response gives an important motive for the optical section, and the limit of object depth. The edge enhancement may be a fatal defeat to the reconstruction of image and sensitive to the conditions of specimen such as slope, irregular reflectivity, shape, etc. That means that the intensity profile for the open loop scanning method should be matched with its response to a perfect mirror as specimen, which can be minimized with the optical sectioning or the optical probe of the closed loop control.

Original languageEnglish (US)
Pages (from-to)58-65
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4564
DOIs
StatePublished - Dec 1 2001
Externally publishedYes
EventOptomechatronic Systems II - Newton, United States
Duration: Oct 29 2001Oct 31 2001

Keywords

  • AO deflector
  • Confocal scanning microscopy (CSM)
  • Image extinction
  • Optical sectioning
  • Unitary lens

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of '3D image reconstruction using optical sectioning in confocal scanning microscopy'. Together they form a unique fingerprint.

  • Cite this