8-Methoxypsoralen suppresses production of ovarian aromatase proteins

Danielle S. McDermott, Patricia B. Hoyer, Moussa M. Diawara

Research output: Contribution to journalReview article

1 Scopus citations

Abstract

8-Methoxypsoralen (8-MOP) is a natural plant biosynthetic metabolite found in produce. Its synthetic form is also widely used in combination with ultraviolet A irradiation in skin photochemotherapy, a procedure called psoralen ultraviolet A (PUVA). This compound has been implicated in a number of adverse reproductive effects in the Wistar rat, including decreased birth rate as a result of decreased ovulations, reduction in levels of circulating 17-β estradiol, and reduction in the granulosa cell population in antral ovarian follicles. The present study examines the potential for decreased production of aromatase (cytochrome P450 19) proteins in the ovaries as a mechanism of 8-MOP-mediated toxicity. Aromatase proteins were measured by Western blotting as a possible origin of decreased estradiol, and estradiol production was measured by radioimmunoassay. Significant decreases in both aromatase proteins and estradiol levels were observed. These results, which are also supported by a decrease in pituitary weight and, possibly, pituitary function, show the potential for 8-MOP-mediated infertility and may explain, in part, adverse reproductive effects reported in humans undergoing PUVA. The findings are consistent with previous observations and with the hypothesis that 8-MOP may affect reproductive function by adversely impacting organs in the hypothalamic-pituitary-gonadal axis.

Original languageEnglish (US)
Pages (from-to)235-245
Number of pages11
JournalToxin Reviews
Volume24
Issue number2
DOIs
StatePublished - Aug 17 2005

Keywords

  • 17-β estradiol
  • CYP19
  • Ovarian toxicity
  • PUVA
  • Psoralens

ASJC Scopus subject areas

  • Toxicology

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