A bicistronic lentiviral vector based on the 1D/2A sequence of foot-and-mouth disease virus expresses proteins stoichiometrically.

Viviana Torres, Lucia Barra, Felipe Garcés, Kely Ordenes, Sergio Leal-Ortiz, Craig C. Garner, Fabian Fernandez, Pedro Zamorano

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Classic IRES sequences are notorious for exerting biased expression in favor of upstream coding regions when placed into polycistronic vectors. Here, we report the development of a bicistronic lentiviral system based on the 1D/2A sequence from the foot-and-mouth disease virus that is able to maintain tightly balanced control of upstream and downstream protein expression for several days at a stoichiometry very closely approaching 1.0. Our results suggest that the 1D/2A sequence can be optimized in an FUGW lentiviral setting to coordinate expression of multiple polypeptides, presenting a potentially valuable tool to signaling network researchers and to the gene therapy community.

Original languageEnglish (US)
Pages (from-to)138-142
Number of pages5
JournalJournal of Biotechnology
Volume146
Issue number3
DOIs
StatePublished - Apr 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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