A bioactive peptide from the transmembrane 5-intracellular loop 3 region of the human 5ht1a receptor

Kenneth Hayataka, Mary-Frances O'Connor, Nancy Kinzler, John T. Weber, Keith K. Parker

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

15 amino acid peptide from the transmembrane 5 - intracellular loop 3 region of the human 5HT1a receptor produced concentration-dependent decreases in agonist binding. This result is consistent with a competitive interaction between peptide, receptor, and G protein at the receptor - G protein interface. Bombesin and a 13 amino acid peptide from the carboxyl terminus region of the receptor were inactive. Additionally, the peptide decreased forskolin-mediated cAMP elevation. Overall, these results suggest that amino acid residues from this region of the receptor are involved in receptor - G protein coupling and that G protein is activated by the receptor.

Original languageEnglish (US)
Pages (from-to)657-660
Number of pages4
JournalBiochemistry and Cell Biology
Volume76
Issue number4
Publication statusPublished - 1998
Externally publishedYes

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Keywords

  • 5HT1a receptor
  • cAMP
  • G protein
  • Loop peptide
  • Serotonin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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