Abstract
The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.
| Original language | English (US) |
|---|---|
| Article number | 193701 |
| Journal | Applied Physics Letters |
| Volume | 101 |
| Issue number | 19 |
| DOIs | |
| State | Published - Nov 5 2012 |
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ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
Cite this
A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture. / Zha, Zhengbao; Jiang, Linan; Dai, Zhifei; Wu, Xiaoyi.
In: Applied Physics Letters, Vol. 101, No. 19, 193701, 05.11.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture
AU - Zha, Zhengbao
AU - Jiang, Linan
AU - Dai, Zhifei
AU - Wu, Xiaoyi
PY - 2012/11/5
Y1 - 2012/11/5
N2 - The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.
AB - The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.
UR - http://www.scopus.com/inward/record.url?scp=84869029257&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84869029257&partnerID=8YFLogxK
U2 - 10.1063/1.4766191
DO - 10.1063/1.4766191
M3 - Article
AN - SCOPUS:84869029257
VL - 101
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 19
M1 - 193701
ER -