A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture

Zhengbao Zha; Linan Jiang; Zhifei Dai; Xiaoyi Wu

doi:10.1063/1.4766191

A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture

Zhengbao Zha, Linan Jiang, Zhifei Dai, Xiaoyi Wu

Aerospace and Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.

Original language	English (US)
Article number	193701
Journal	Applied Physics Letters
Volume	101
Issue number	19
DOIs	https://doi.org/10.1063/1.4766191
State	Published - Nov 5 2012

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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title = "A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture",

abstract = "The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.",

author = "Zhengbao Zha and Linan Jiang and Zhifei Dai and Xiaoyi Wu",

note = "Funding Information: This work was supported by the US National Institutes of Health (EB009160) and National Science Foundation (CMMI0855890), and National Science Foundation of China (2007AA03Z316, 30970829). ",

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AU - Zha, Zhengbao

AU - Jiang, Linan

AU - Dai, Zhifei

AU - Wu, Xiaoyi

N1 - Funding Information: This work was supported by the US National Institutes of Health (EB009160) and National Science Foundation (CMMI0855890), and National Science Foundation of China (2007AA03Z316, 30970829).

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N2 - The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.

AB - The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.

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A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture

Abstract

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