A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture

Zhengbao Zha, Linan Jiang, Zhifei Dai, Xiaoyi Wu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.

Original languageEnglish (US)
Article number193701
JournalApplied Physics Letters
Volume101
Issue number19
DOIs
StatePublished - Nov 5 2012

Fingerprint

biomimetics
antibodies
immobilization
silanes
lipids
membranes
cells
molecules
inserts
hydration
microscopy

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture. / Zha, Zhengbao; Jiang, Linan; Dai, Zhifei; Wu, Xiaoyi.

In: Applied Physics Letters, Vol. 101, No. 19, 193701, 05.11.2012.

Research output: Contribution to journalArticle

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