A cellulose acetate electrophoretic procedure evaluated for quantitation of high-density lipoprotein cholesterol

Ronald B Schifman, P. F. Brumbaugh, D. Grover, P. R. Finley, E. J. Harrow

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We evaluated the performance of a commercially available cellulose acetate electrophoretic method for quantitating high-density lipoprotein cholesterol (I) in serum by comparing it to a method involving precipitation with dextran sulfate-500/Mg 2+. In both methods, enzymic reagents are used for cholesterol measurement. For electrophoretic measurement of I the mean intramembrane CV was 4.1% (at 220 to 360 mg/L) and the intermembrane CV ranged from 12.2 to 21.0% (at 220 to 880 mg/L). Interassay precision was significantly better for the precipitation method (CV = 3.9% at 390 mg/L). The electrophoretic procedure demonstrated significant measurement bias, both at high and low I concentrations. However, low-density lipoprotein cholesterol, measured electrophoretically, correlated well with its calculated concentrations obtained by the precipitation method. Measurements of I by this electrophoretic procedure did not achieve the accuracy and reproducibility that have been demonstrated for precipitation methods and that are necessary for reliable clinical interpretation of results for I.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalClinical Chemistry
Volume27
Issue number1
StatePublished - 1981

Fingerprint

HDL Cholesterol
Dextran Sulfate
LDL Cholesterol
Cholesterol
acetylcellulose
Serum

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

A cellulose acetate electrophoretic procedure evaluated for quantitation of high-density lipoprotein cholesterol. / Schifman, Ronald B; Brumbaugh, P. F.; Grover, D.; Finley, P. R.; Harrow, E. J.

In: Clinical Chemistry, Vol. 27, No. 1, 1981, p. 175-178.

Research output: Contribution to journalArticle

Schifman, Ronald B ; Brumbaugh, P. F. ; Grover, D. ; Finley, P. R. ; Harrow, E. J. / A cellulose acetate electrophoretic procedure evaluated for quantitation of high-density lipoprotein cholesterol. In: Clinical Chemistry. 1981 ; Vol. 27, No. 1. pp. 175-178.
@article{f09d7866634a4b01a48df6c330b2bc18,
title = "A cellulose acetate electrophoretic procedure evaluated for quantitation of high-density lipoprotein cholesterol",
abstract = "We evaluated the performance of a commercially available cellulose acetate electrophoretic method for quantitating high-density lipoprotein cholesterol (I) in serum by comparing it to a method involving precipitation with dextran sulfate-500/Mg 2+. In both methods, enzymic reagents are used for cholesterol measurement. For electrophoretic measurement of I the mean intramembrane CV was 4.1{\%} (at 220 to 360 mg/L) and the intermembrane CV ranged from 12.2 to 21.0{\%} (at 220 to 880 mg/L). Interassay precision was significantly better for the precipitation method (CV = 3.9{\%} at 390 mg/L). The electrophoretic procedure demonstrated significant measurement bias, both at high and low I concentrations. However, low-density lipoprotein cholesterol, measured electrophoretically, correlated well with its calculated concentrations obtained by the precipitation method. Measurements of I by this electrophoretic procedure did not achieve the accuracy and reproducibility that have been demonstrated for precipitation methods and that are necessary for reliable clinical interpretation of results for I.",
author = "Schifman, {Ronald B} and Brumbaugh, {P. F.} and D. Grover and Finley, {P. R.} and Harrow, {E. J.}",
year = "1981",
language = "English (US)",
volume = "27",
pages = "175--178",
journal = "Clinical Chemistry",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "1",

}

TY - JOUR

T1 - A cellulose acetate electrophoretic procedure evaluated for quantitation of high-density lipoprotein cholesterol

AU - Schifman, Ronald B

AU - Brumbaugh, P. F.

AU - Grover, D.

AU - Finley, P. R.

AU - Harrow, E. J.

PY - 1981

Y1 - 1981

N2 - We evaluated the performance of a commercially available cellulose acetate electrophoretic method for quantitating high-density lipoprotein cholesterol (I) in serum by comparing it to a method involving precipitation with dextran sulfate-500/Mg 2+. In both methods, enzymic reagents are used for cholesterol measurement. For electrophoretic measurement of I the mean intramembrane CV was 4.1% (at 220 to 360 mg/L) and the intermembrane CV ranged from 12.2 to 21.0% (at 220 to 880 mg/L). Interassay precision was significantly better for the precipitation method (CV = 3.9% at 390 mg/L). The electrophoretic procedure demonstrated significant measurement bias, both at high and low I concentrations. However, low-density lipoprotein cholesterol, measured electrophoretically, correlated well with its calculated concentrations obtained by the precipitation method. Measurements of I by this electrophoretic procedure did not achieve the accuracy and reproducibility that have been demonstrated for precipitation methods and that are necessary for reliable clinical interpretation of results for I.

AB - We evaluated the performance of a commercially available cellulose acetate electrophoretic method for quantitating high-density lipoprotein cholesterol (I) in serum by comparing it to a method involving precipitation with dextran sulfate-500/Mg 2+. In both methods, enzymic reagents are used for cholesterol measurement. For electrophoretic measurement of I the mean intramembrane CV was 4.1% (at 220 to 360 mg/L) and the intermembrane CV ranged from 12.2 to 21.0% (at 220 to 880 mg/L). Interassay precision was significantly better for the precipitation method (CV = 3.9% at 390 mg/L). The electrophoretic procedure demonstrated significant measurement bias, both at high and low I concentrations. However, low-density lipoprotein cholesterol, measured electrophoretically, correlated well with its calculated concentrations obtained by the precipitation method. Measurements of I by this electrophoretic procedure did not achieve the accuracy and reproducibility that have been demonstrated for precipitation methods and that are necessary for reliable clinical interpretation of results for I.

UR - http://www.scopus.com/inward/record.url?scp=0019364612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019364612&partnerID=8YFLogxK

M3 - Article

C2 - 6160925

AN - SCOPUS:0019364612

VL - 27

SP - 175

EP - 178

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 1

ER -