A cellulose acetate electrophoretic procedure evaluated for quantitation of high-density lipoprotein cholesterol

R. B. Schifman, P. F. Brumbaugh, D. Grover, P. R. Finley, E. J. Harrow

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We evaluated the performance of a commercially available cellulose acetate electrophoretic method for quantitating high-density lipoprotein cholesterol (I) in serum by comparing it to a method involving precipitation with dextran sulfate-500/Mg2+. In both methods, enzymic reagents are used for cholesterol measurement. For electrophoretic measurement of I the mean intramembrane CV was 4.1% (at 220 to 360 mg/L) and the intermembrane CV ranged from 12.2 to 21.0% (at 220 to 880 mg/L). Interassay precision was significantly better for the precipitation method (CV = 3.9% at 390 mg/L). The electrophoretic procedure demonstrated significant measurement bias, both at high and low I concentrations. However, low-density lipoprotein cholesterol, measured electrophoretically, correlated well with its calculated concentrations obtained by the precipitation method. Measurements of I by this electrophoretic procedure did not achieve the accuracy and reproducibility that have been demonstrated for precipitation methods and that are necessary for reliable clinical interpretation of results for I.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalUnknown Journal
Volume27
Issue number1
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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