A Characterization of the Manduca sexta Serotonin Receptors in the Context of Olfactory Neuromodulation

Andrew M. Dacks, Vincenzina Reale, Yeli Pi, Wujie Zhang, Joel B. Dacks, Alan J Nighorn, Peter D. Evans

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Neuromodulation, the alteration of individual neuron response properties, has dramatic consequences for neural network function and is a phenomenon observed across all brain regions and taxa. However, the mechanisms underlying neuromodulation are made complex by the diversity of neuromodulatory receptors expressed within a neural network. In this study we begin to examine the receptor basis for serotonergic neuromodulation in the antennal lobe of Manduca sexta. To this end we cloned all four known insect serotonin receptor types from Manduca (the Ms5HTRs). We used phylogenetic analyses to classify the Ms5HTRs and to establish their relationships to other insect serotonin receptors, other insect amine receptors and the vertebrate serotonin receptors. Pharmacological assays demonstrated that each Ms5HTR was selective for serotonin over other endogenous amines and that serotonin had a similar potency at all four Ms5HTRs. The pharmacological assays also identified several agonists and antagonists of the different Ms5HTRs. Finally, we found that the Ms5HT1A receptor was expressed in a subpopulation of GABAergic local interneurons suggesting that the Ms5HTRs are likely expressed heterogeneously within the antennal lobe based on functional neuronal subtype.

Original languageEnglish (US)
Article numbere69422
JournalPLoS One
Volume8
Issue number7
DOIs
StatePublished - Jul 29 2013

Fingerprint

Manduca
Serotonin Receptors
Manduca sexta
Insects
antennal lobe
receptors
Amines
Assays
Serotonin
amines
serotonin
Pharmacology
neural networks
insects
Neural networks
Interneurons
Neurons
Vertebrates
Brain
interneurons

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A Characterization of the Manduca sexta Serotonin Receptors in the Context of Olfactory Neuromodulation. / Dacks, Andrew M.; Reale, Vincenzina; Pi, Yeli; Zhang, Wujie; Dacks, Joel B.; Nighorn, Alan J; Evans, Peter D.

In: PLoS One, Vol. 8, No. 7, e69422, 29.07.2013.

Research output: Contribution to journalArticle

Dacks, Andrew M. ; Reale, Vincenzina ; Pi, Yeli ; Zhang, Wujie ; Dacks, Joel B. ; Nighorn, Alan J ; Evans, Peter D. / A Characterization of the Manduca sexta Serotonin Receptors in the Context of Olfactory Neuromodulation. In: PLoS One. 2013 ; Vol. 8, No. 7.
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