A compact LED-based module for DNA capillary electrophoresis

C. Hurth, R. Lenigk, Frederic Zenhausern

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A setup consisting of a bifurcated optical fiber made from high-transmission fused-silica cores with relatively high numerical apertures (NA=0.22), high-power cyan light-emitting diodes (LEDs) and Peltier cooling elements controlled by a proportional-integrative-derivative (PID) module is introduced to replace bulky, power- consuming lasers conventionally used in laser induced fluorescence (LIF) microchip capillary electrophoresis (μCE). The output fiber beam size, divergence, power distribution and power stability over time are documented. A modified epifluorescence microscope arrangement is used in conjunction with a compact fixed spectrometer aligned with a cooled charge-coupled device (CCD) camera for added sensitivity. Fluorescent dyes such as fluorescein, 6-carboxyfluorescein (6-FAM) and rhodamine B can be detected in cyclic olefin copolymer (COC) and glass microchannels at submicromolar levels. A single-stranded DNA oligonucleotide (10-mer) labeled with 6-FAM is also detected with reasonable signal-to-noise ratio when electrophoretically migrated at 100 V/cm. The compact LED excitation system presented herein will allow using capillary electrophoresis for DNA detection in compact mobile devices.

Original languageEnglish (US)
Pages (from-to)693-699
Number of pages7
JournalApplied Physics B: Lasers and Optics
Volume93
Issue number2-3
DOIs
StatePublished - Nov 2008

Fingerprint

electrophoresis
light emitting diodes
deoxyribonucleic acid
modules
oligonucleotides
numerical aperture
rhodamine
microchannels
laser induced fluorescence
alkenes
charge coupled devices
copolymers
divergence
signal to noise ratios
optical fibers
dyes
cameras
microscopes
spectrometers
silicon dioxide

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physics and Astronomy (miscellaneous)

Cite this

A compact LED-based module for DNA capillary electrophoresis. / Hurth, C.; Lenigk, R.; Zenhausern, Frederic.

In: Applied Physics B: Lasers and Optics, Vol. 93, No. 2-3, 11.2008, p. 693-699.

Research output: Contribution to journalArticle

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