A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins

Hideaki Takata, Susumu Uchiyama, Naoko Nakamura, Shoko Nakashima, Shouhei Kobayashi, Takefumi Sone, Sumiko Kimura, Sunshine Lahmers, Hendrikus "Henk" Granzier, Siegfried Labeit, Sachihiro Matsunaga, Kiichi Fukui

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A comparative proteome analysis of human metaphase chromosomes between a typical epithelial-like cell, HeLa S3, and a lymphoma-type cell, BALL-1, was performed. One-dimensional (1-D) SDS-PAGE and radical-free and highly reducing two-dimensional electrophoresis (RFHR 2-DE) detected more than 200 proteins from chromosomes isolated from HeLa S3 cells, among which 189 proteins were identified by mass spectrometry (MS). Consistent with our recent four-layer structural model of a metaphase chromosome, all the identified proteins were grouped into four distinct levels of abundance. Both HeLa S3 and BALL-1 chromosomes contained specific sets of abundant chromosome structural and peripheral proteins in addition to less abundant chromosome coating proteins (CCPs). Furthermore, titin array analysis and a proteome analysis of the ultra-high molecular mass region indicated an absence of titin with their molecular weight (MW) more than 1000 kDa. Consequently, the present proteome analyses together with previous information on chromosome proteins provide the comprehensive list of proteins essential for the metaphase chromosome architecture.

Original languageEnglish (US)
Pages (from-to)269-284
Number of pages16
JournalGenes to Cells
Volume12
Issue number3
DOIs
StatePublished - Mar 2007
Externally publishedYes

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Human Chromosomes
Proteome
Metaphase
Chromosomes
Cell Line
Proteins
Connectin
Chromosomes, Human, Pair 1
Structural Models
HeLa Cells
Free Radicals
Electrophoresis
Polyacrylamide Gel Electrophoresis
Lymphoma
Mass Spectrometry
Molecular Weight
Epithelial Cells

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins. / Takata, Hideaki; Uchiyama, Susumu; Nakamura, Naoko; Nakashima, Shoko; Kobayashi, Shouhei; Sone, Takefumi; Kimura, Sumiko; Lahmers, Sunshine; Granzier, Hendrikus "Henk"; Labeit, Siegfried; Matsunaga, Sachihiro; Fukui, Kiichi.

In: Genes to Cells, Vol. 12, No. 3, 03.2007, p. 269-284.

Research output: Contribution to journalArticle

Takata, H, Uchiyama, S, Nakamura, N, Nakashima, S, Kobayashi, S, Sone, T, Kimura, S, Lahmers, S, Granzier, HH, Labeit, S, Matsunaga, S & Fukui, K 2007, 'A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins', Genes to Cells, vol. 12, no. 3, pp. 269-284. https://doi.org/10.1111/j.1365-2443.2007.01051.x
Takata, Hideaki ; Uchiyama, Susumu ; Nakamura, Naoko ; Nakashima, Shoko ; Kobayashi, Shouhei ; Sone, Takefumi ; Kimura, Sumiko ; Lahmers, Sunshine ; Granzier, Hendrikus "Henk" ; Labeit, Siegfried ; Matsunaga, Sachihiro ; Fukui, Kiichi. / A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins. In: Genes to Cells. 2007 ; Vol. 12, No. 3. pp. 269-284.
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