A cysteine-string protein is expressed in retina and brain of drosophila

Konrad E Zinsmaier, Alois Hofbauer, Gertrud Heimbeck, Gert O. Pflugfelder, Sigrid Buchner, Erich Buchner

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Antibodies can be used to identify tissue- and stage-specifically expressed genes. A monoclonal antibody MAB ab49 from a hybridoma library screened for immunohistochemical staining in the adult nervous system of Drosophila melanogaster was found to selectively bind to all neuropil regions and to synaptic boutons of motor neurons. In Western blots of homogenized brains the antibody recognizes two proteins of 32 and 34 kD. Using this antibody we have isolated seven cDNA clones that derive from two polyadeny-lated mRNA splice variants of a gene located at 79E1-2 on polytene chromosomes. The two mRNAs code for two inferred proteins of 249 and 223 amino acids, respectively, which are identical except for their C-terminals and a central deletion of 21 amino acids in the second protein. Both contain a contiguous string of 11 cysteine residues. In situ hybridization to frozen head sections detects expression of this gene in retina and neuronal perikarya. The 32 and 34 kD brain proteins that presumably are localized predominantly in synaptic terminals of photoreceptors and most if not all neurons may correspond to two variant cysteinestring proteins as they are of similar molecular weight and share an antigenic binding site for MAB ab49.

Original languageEnglish (US)
Pages (from-to)15-29
Number of pages15
JournalJournal of Neurogenetics
Volume7
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Drosophila
Retina
Brain
Presynaptic Terminals
Proteins
Antibodies
Polytene Chromosomes
Amino Acids
Messenger RNA
Neuropil
Hybridomas
Frozen Sections
Motor Neurons
Drosophila melanogaster
Nervous System
Genes
In Situ Hybridization
Cysteine
Complementary DNA
Clone Cells

Keywords

  • Brain-specific protein
  • Drosophila melanogaster
  • Expression library
  • Monoclonal antibody
  • Splice variants

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Genetics
  • Developmental Biology
  • Neuroscience(all)

Cite this

Zinsmaier, K. E., Hofbauer, A., Heimbeck, G., Pflugfelder, G. O., Buchner, S., & Buchner, E. (1990). A cysteine-string protein is expressed in retina and brain of drosophila. Journal of Neurogenetics, 7(1), 15-29. https://doi.org/10.3109/01677069009084150

A cysteine-string protein is expressed in retina and brain of drosophila. / Zinsmaier, Konrad E; Hofbauer, Alois; Heimbeck, Gertrud; Pflugfelder, Gert O.; Buchner, Sigrid; Buchner, Erich.

In: Journal of Neurogenetics, Vol. 7, No. 1, 1990, p. 15-29.

Research output: Contribution to journalArticle

Zinsmaier, KE, Hofbauer, A, Heimbeck, G, Pflugfelder, GO, Buchner, S & Buchner, E 1990, 'A cysteine-string protein is expressed in retina and brain of drosophila', Journal of Neurogenetics, vol. 7, no. 1, pp. 15-29. https://doi.org/10.3109/01677069009084150
Zinsmaier, Konrad E ; Hofbauer, Alois ; Heimbeck, Gertrud ; Pflugfelder, Gert O. ; Buchner, Sigrid ; Buchner, Erich. / A cysteine-string protein is expressed in retina and brain of drosophila. In: Journal of Neurogenetics. 1990 ; Vol. 7, No. 1. pp. 15-29.
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