A DNA polymerase stop assay for G-quadruplex-interactive compounds

Haiyong Han, Laurence H. Hurley, Miguel Salazar

Research output: Contribution to journalArticle

182 Scopus citations

Abstract

We have developed and characterized an assay for G-quadruplex-interactive compounds that makes use of the fact that G-rich DNA templates present obstacles to DNA synthesis by DNA polymerases. Using Taq DNA polymerase and the G-quadruplex binding 2,6-diamidoanthraquinone BSU-1051, we find that BSU-1051 leads to enhanced arrest of DNA synthesis in the presence of K+ by stabilizing an intramolecular G-quadruplex structure formed by four repeats of either TTGGGG or TTAGGG in the template strand. The data provide additional evidence that BSU-1051 modulates telomerase activity by stabilization of telomeric G-quadruplex DNA and point to a polymerase arrest assay as a sensitive method for screening for G-quadruplex-interactive agents with potential clinical utility.

Original languageEnglish (US)
Pages (from-to)537-542
Number of pages6
JournalNucleic acids research
Volume27
Issue number2
DOIs
StatePublished - Jan 15 1999
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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