A DNA polymerase stop assay for G-quadruplex-interactive compounds

Haiyong Han, Laurence Hurley, Miguel Salazar

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

We have developed and characterized an assay for G-quadruplex-interactive compounds that makes use of the fact that G-rich DNA templates present obstacles to DNA synthesis by DNA polymerases. Using Taq DNA polymerase and the G-quadruplex binding 2,6-diamidoanthraquinone BSU-1051, we find that BSU-1051 leads to enhanced arrest of DNA synthesis in the presence of K+ by stabilizing an intramolecular G-quadruplex structure formed by four repeats of either TTGGGG or TTAGGG in the template strand. The data provide additional evidence that BSU-1051 modulates telomerase activity by stabilization of telomeric G-quadruplex DNA and point to a polymerase arrest assay as a sensitive method for screening for G-quadruplex-interactive agents with potential clinical utility.

Original languageEnglish (US)
Pages (from-to)537-542
Number of pages6
JournalNucleic Acids Research
Volume27
Issue number2
DOIs
StatePublished - Jan 15 1999
Externally publishedYes

Fingerprint

G-Quadruplexes
DNA-Directed DNA Polymerase
DNA
Taq Polymerase
Telomerase
BSU 1051

ASJC Scopus subject areas

  • Genetics

Cite this

A DNA polymerase stop assay for G-quadruplex-interactive compounds. / Han, Haiyong; Hurley, Laurence; Salazar, Miguel.

In: Nucleic Acids Research, Vol. 27, No. 2, 15.01.1999, p. 537-542.

Research output: Contribution to journalArticle

Han, Haiyong ; Hurley, Laurence ; Salazar, Miguel. / A DNA polymerase stop assay for G-quadruplex-interactive compounds. In: Nucleic Acids Research. 1999 ; Vol. 27, No. 2. pp. 537-542.
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