A dual modality fluorescence confocal and optical coherence tomography microendoscope

Houssine Makhlouf, Andrew R Rouse, Arthur F Gmitro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We demonstrate the implementation of a Fourier domain optical coherence tomography (OCT) imaging system incorporated into the optical train of a fluorescence confocal microendoscope. The slit-scanning confocal system has been presented previously and achieves 3μm lateral resolution and 25μm axial resolution over a field of view of 430μm. Its multi-spectral mode of operation captures images with 6nm average spectral resolution. To incorporate OCT imaging, a common-path interferometer is made with a super luminescent diode and a reference coverslip located at the distal end of the fiber bundle catheter. The infrared diode spectral width allows a theoretical OCT axial resolution of 12.9μm. Light from the reference and sample combine, and a diffraction grating produces a spectral interferogram on the same 2D CCD camera used for confocal microendoscopic imaging. OCT depth information is recovered by a Fourier transform along the spectral dispersion direction. Proper operation of the system scan mirrors allows rapid switching between confocal and OCT imaging modes. The OCT extension takes advantage of the slit geometry, so that a 2D image is acquired without scanning. Combining confocal and OCT imaging modalities provides a more comprehensive view of tissue and the potential to improve disease diagnosis. A preliminary bench-top system design and imaging results are presented.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7558
DOIs
StatePublished - 2010
EventEndoscopic Microscopy V - San Francisco, CA, United States
Duration: Jan 24 2010Jan 25 2010

Other

OtherEndoscopic Microscopy V
CountryUnited States
CitySan Francisco, CA
Period1/24/101/25/10

Fingerprint

Optical tomography
Optical Coherence Tomography
tomography
Fluorescence
fluorescence
Imaging techniques
slits
Diodes
diodes
Scanning
scanning
Catheters
Spectral resolution
Diffraction gratings
Fourier Analysis
CCD cameras
gratings (spectra)
systems engineering
spectral resolution
Imaging systems

Keywords

  • Confocal
  • Fiber-bundle
  • Microendoscopy
  • Multi-spectral
  • Optical coherence tomography
  • Slit-scan
  • Super luminescent diode

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Makhlouf, H., Rouse, A. R., & Gmitro, A. F. (2010). A dual modality fluorescence confocal and optical coherence tomography microendoscope. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7558). [75580K] https://doi.org/10.1117/12.842780

A dual modality fluorescence confocal and optical coherence tomography microendoscope. / Makhlouf, Houssine; Rouse, Andrew R; Gmitro, Arthur F.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7558 2010. 75580K.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Makhlouf, H, Rouse, AR & Gmitro, AF 2010, A dual modality fluorescence confocal and optical coherence tomography microendoscope. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7558, 75580K, Endoscopic Microscopy V, San Francisco, CA, United States, 1/24/10. https://doi.org/10.1117/12.842780
Makhlouf H, Rouse AR, Gmitro AF. A dual modality fluorescence confocal and optical coherence tomography microendoscope. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7558. 2010. 75580K https://doi.org/10.1117/12.842780
Makhlouf, Houssine ; Rouse, Andrew R ; Gmitro, Arthur F. / A dual modality fluorescence confocal and optical coherence tomography microendoscope. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7558 2010.
@inproceedings{2e32446ba8e44d7c953c962fc6b74b94,
title = "A dual modality fluorescence confocal and optical coherence tomography microendoscope",
abstract = "We demonstrate the implementation of a Fourier domain optical coherence tomography (OCT) imaging system incorporated into the optical train of a fluorescence confocal microendoscope. The slit-scanning confocal system has been presented previously and achieves 3μm lateral resolution and 25μm axial resolution over a field of view of 430μm. Its multi-spectral mode of operation captures images with 6nm average spectral resolution. To incorporate OCT imaging, a common-path interferometer is made with a super luminescent diode and a reference coverslip located at the distal end of the fiber bundle catheter. The infrared diode spectral width allows a theoretical OCT axial resolution of 12.9μm. Light from the reference and sample combine, and a diffraction grating produces a spectral interferogram on the same 2D CCD camera used for confocal microendoscopic imaging. OCT depth information is recovered by a Fourier transform along the spectral dispersion direction. Proper operation of the system scan mirrors allows rapid switching between confocal and OCT imaging modes. The OCT extension takes advantage of the slit geometry, so that a 2D image is acquired without scanning. Combining confocal and OCT imaging modalities provides a more comprehensive view of tissue and the potential to improve disease diagnosis. A preliminary bench-top system design and imaging results are presented.",
keywords = "Confocal, Fiber-bundle, Microendoscopy, Multi-spectral, Optical coherence tomography, Slit-scan, Super luminescent diode",
author = "Houssine Makhlouf and Rouse, {Andrew R} and Gmitro, {Arthur F}",
year = "2010",
doi = "10.1117/12.842780",
language = "English (US)",
isbn = "9780819479549",
volume = "7558",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

}

TY - GEN

T1 - A dual modality fluorescence confocal and optical coherence tomography microendoscope

AU - Makhlouf, Houssine

AU - Rouse, Andrew R

AU - Gmitro, Arthur F

PY - 2010

Y1 - 2010

N2 - We demonstrate the implementation of a Fourier domain optical coherence tomography (OCT) imaging system incorporated into the optical train of a fluorescence confocal microendoscope. The slit-scanning confocal system has been presented previously and achieves 3μm lateral resolution and 25μm axial resolution over a field of view of 430μm. Its multi-spectral mode of operation captures images with 6nm average spectral resolution. To incorporate OCT imaging, a common-path interferometer is made with a super luminescent diode and a reference coverslip located at the distal end of the fiber bundle catheter. The infrared diode spectral width allows a theoretical OCT axial resolution of 12.9μm. Light from the reference and sample combine, and a diffraction grating produces a spectral interferogram on the same 2D CCD camera used for confocal microendoscopic imaging. OCT depth information is recovered by a Fourier transform along the spectral dispersion direction. Proper operation of the system scan mirrors allows rapid switching between confocal and OCT imaging modes. The OCT extension takes advantage of the slit geometry, so that a 2D image is acquired without scanning. Combining confocal and OCT imaging modalities provides a more comprehensive view of tissue and the potential to improve disease diagnosis. A preliminary bench-top system design and imaging results are presented.

AB - We demonstrate the implementation of a Fourier domain optical coherence tomography (OCT) imaging system incorporated into the optical train of a fluorescence confocal microendoscope. The slit-scanning confocal system has been presented previously and achieves 3μm lateral resolution and 25μm axial resolution over a field of view of 430μm. Its multi-spectral mode of operation captures images with 6nm average spectral resolution. To incorporate OCT imaging, a common-path interferometer is made with a super luminescent diode and a reference coverslip located at the distal end of the fiber bundle catheter. The infrared diode spectral width allows a theoretical OCT axial resolution of 12.9μm. Light from the reference and sample combine, and a diffraction grating produces a spectral interferogram on the same 2D CCD camera used for confocal microendoscopic imaging. OCT depth information is recovered by a Fourier transform along the spectral dispersion direction. Proper operation of the system scan mirrors allows rapid switching between confocal and OCT imaging modes. The OCT extension takes advantage of the slit geometry, so that a 2D image is acquired without scanning. Combining confocal and OCT imaging modalities provides a more comprehensive view of tissue and the potential to improve disease diagnosis. A preliminary bench-top system design and imaging results are presented.

KW - Confocal

KW - Fiber-bundle

KW - Microendoscopy

KW - Multi-spectral

KW - Optical coherence tomography

KW - Slit-scan

KW - Super luminescent diode

UR - http://www.scopus.com/inward/record.url?scp=77951731294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951731294&partnerID=8YFLogxK

U2 - 10.1117/12.842780

DO - 10.1117/12.842780

M3 - Conference contribution

AN - SCOPUS:77951731294

SN - 9780819479549

VL - 7558

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ER -