A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis

Kenneth A Feldmann, M. David Marks, Michael L. Christianson, Ralph S. Quatrano

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

Most plant genes that control complex traits of tissues, organs, and whole plants are uncharacterized. Plant height, structure of reproductive organs, seed development and germination, for example, are traits of great agronomic importance. However, in the absence of knowledge of the gene products, current molecular approaches to isolate these important genes are limited. Infection of germinating seeds of Arabidopsis thaliana with Agrobacterium results in transformed lines in which the integrated T-DNA from Agrobacterium and its associated kanamycin-resistance trait cosegregate with stable, phenotypic alterations. A survey of 136 transformed lines produced plants segregating in a manner consistent with Mendelian predictions for phenotypes altered in height, flower structure, trichomes, gametogenesis, embryogenesis, and seedling development. This report is the characterization of a dwarf mutant in which the phenotype is inherited as a single recessive nuclear mutation that cosegregates with both the kanamycin-resistance trait and the T-DNA insert.

Original languageEnglish (US)
Pages (from-to)1351-1354
Number of pages4
JournalScience
Volume243
Issue number4896
StatePublished - 1989
Externally publishedYes

Fingerprint

mutagenesis
Arabidopsis
mutants
DNA
kanamycin
Agrobacterium
phenotype
genes
gametogenesis
seed development
trichomes
seed germination
embryogenesis
Arabidopsis thaliana
mutation
flowers
prediction
seedlings
seeds
infection

ASJC Scopus subject areas

  • General

Cite this

Feldmann, K. A., Marks, M. D., Christianson, M. L., & Quatrano, R. S. (1989). A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. Science, 243(4896), 1351-1354.

A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. / Feldmann, Kenneth A; Marks, M. David; Christianson, Michael L.; Quatrano, Ralph S.

In: Science, Vol. 243, No. 4896, 1989, p. 1351-1354.

Research output: Contribution to journalArticle

Feldmann, KA, Marks, MD, Christianson, ML & Quatrano, RS 1989, 'A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis', Science, vol. 243, no. 4896, pp. 1351-1354.
Feldmann KA, Marks MD, Christianson ML, Quatrano RS. A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. Science. 1989;243(4896):1351-1354.
Feldmann, Kenneth A ; Marks, M. David ; Christianson, Michael L. ; Quatrano, Ralph S. / A dwarf mutant of Arabidopsis generated by T-DNA insertion mutagenesis. In: Science. 1989 ; Vol. 243, No. 4896. pp. 1351-1354.
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