A free radical scavenger (Lazaroid U75412E) attenuates tumor necrosis factor-alpha generation in a rabbit model of smoke-induced lung injury

Shengjun Wang, Robert Clark Lantz, Evelyn D. Rider, Guan Jie Chen, Veronica Breceda, Allison M. Hays, Raymond F. Robledo, Brian J. Tollinger, S. V R Dinesh, Mark L. Witten

Research output: Contribution to journalArticle

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Abstract

The lazaroid (21-aminosteroid) analogue U75412E was evaluated in rabbits exposed to diesel fuel-polycarbonate plastic smoke. Inhalation of a total of 4.6 mg U75412E aerosolized at a rate of 1.53 mg/min for 3 min before or after smoke significantly prevented or limited the extent of alveolar hypoventilation, interstitial edema, and tumor necrosis factor-α (TNF-α) by pulmonary alveolar macrophages (PAM) ex vivo observed at 2 h. The smoke-induced changes in wet lung/body weight ratios and the production of superoxide (O2-) by PAM ex vivo were also attenuated by the drug treatment after smoke exposure (p < 0.05). This study suggests that lazaroids may ameliorate the oxygen-radical-initiated cytokine processes and inflammation cascade as a result of the smoke insult.

Original languageEnglish (US)
Pages (from-to)358-363
Number of pages6
JournalRespiration
Volume64
Issue number5
StatePublished - Sep 1997

Fingerprint

Free Radical Scavengers
Lung Injury
Smoke
Alveolar Macrophages
Tumor Necrosis Factor-alpha
Rabbits
Hypoventilation
Gasoline
Pulmonary Edema
Superoxides
Inhalation
Reactive Oxygen Species
Edema
Body Weight
U 75412E
Cytokines
Inflammation
Pharmaceutical Preparations

Keywords

  • Acute lung injury
  • Lazaroid
  • Pulmonary alveolar macrophages
  • Smoke
  • Superoxide
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology

Cite this

A free radical scavenger (Lazaroid U75412E) attenuates tumor necrosis factor-alpha generation in a rabbit model of smoke-induced lung injury. / Wang, Shengjun; Lantz, Robert Clark; Rider, Evelyn D.; Chen, Guan Jie; Breceda, Veronica; Hays, Allison M.; Robledo, Raymond F.; Tollinger, Brian J.; Dinesh, S. V R; Witten, Mark L.

In: Respiration, Vol. 64, No. 5, 09.1997, p. 358-363.

Research output: Contribution to journalArticle

Wang, S, Lantz, RC, Rider, ED, Chen, GJ, Breceda, V, Hays, AM, Robledo, RF, Tollinger, BJ, Dinesh, SVR & Witten, ML 1997, 'A free radical scavenger (Lazaroid U75412E) attenuates tumor necrosis factor-alpha generation in a rabbit model of smoke-induced lung injury', Respiration, vol. 64, no. 5, pp. 358-363.
Wang, Shengjun ; Lantz, Robert Clark ; Rider, Evelyn D. ; Chen, Guan Jie ; Breceda, Veronica ; Hays, Allison M. ; Robledo, Raymond F. ; Tollinger, Brian J. ; Dinesh, S. V R ; Witten, Mark L. / A free radical scavenger (Lazaroid U75412E) attenuates tumor necrosis factor-alpha generation in a rabbit model of smoke-induced lung injury. In: Respiration. 1997 ; Vol. 64, No. 5. pp. 358-363.
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