A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate

J. Antoinette Killian, Theodore "Ted" Trouard, Denise V. Greathouse, Vladimir Chupin, Göran Lindblom

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.

Original languageEnglish (US)
Pages (from-to)161-165
Number of pages5
JournalFEBS Letters
Volume348
Issue number2
DOIs
StatePublished - Jul 11 1994
Externally publishedYes

Fingerprint

Micelles
Sodium Dodecyl Sulfate
Peptides
Trifluoroethanol
Freeze Drying
Water
Detergents
Powders

Keywords

  • Circular dichroism
  • Gramicidin
  • Micelle
  • Peptide structure
  • Signal peptide
  • Two-dimensional NMR

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate. / Killian, J. Antoinette; Trouard, Theodore "Ted"; Greathouse, Denise V.; Chupin, Vladimir; Lindblom, Göran.

In: FEBS Letters, Vol. 348, No. 2, 11.07.1994, p. 161-165.

Research output: Contribution to journalArticle

Killian, J. Antoinette ; Trouard, Theodore "Ted" ; Greathouse, Denise V. ; Chupin, Vladimir ; Lindblom, Göran. / A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate. In: FEBS Letters. 1994 ; Vol. 348, No. 2. pp. 161-165.
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