A general procedure for evaluation of immunological relevance of synthetic peptides: Peptides synthesized on paper in enzyme-linked immunosorbent assay

Viktor Krchňák, Josef Vágner, Jan Novák, Alena Suchánková, Jaroslav Roubal

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (Whatman 3MM paper). Paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. The method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of HIV, the Epstein-Barr virus-determined nuclear antigen-1 and VCA proteins of the Epstein-Barr virus, and the early region of human papillomavirus type 11.

Original languageEnglish (US)
Pages (from-to)80-83
Number of pages4
JournalAnalytical Biochemistry
Volume189
Issue number1
DOIs
StatePublished - Aug 15 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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