A genetic strategy to overcome the senescence of primary meningioma cell cultures

Gilson S. Baia, Alison L. Slocum, Jeanette D. Hyer, Anjan Misra, Nouzhan Sehati, Scott R. VandenBerg, Burt G.F. Feuerstein, Dennis F. Deen, Michael W. McDermott, Anita Lal

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Even though meningiomas are the second most common brain tumor in adults, little is known about the molecular basis of their growth and development. The lack of suitable cell culture model systems is an impediment to this understanding. Most studies on meningiomas rely on primary, early passage cell lines that eventually senesce or a few established cell lines that have been derived from aggressive variants of meningiomas. We have isolated three primary meningioma cell lines that are negative for telomerase activity. We can overcome the senescence of a Grade III derived meningioma cell line by expressing the telomerase catalytic subunit (hTERT), whereas Grade I meningioma cell lines require the expression of the human papillomavirus E6 and E7 oncogenes in conjunction with hTERT. Meningioma cell lines, immortalized in this manner, maintain their pre-transfection morphology and form colonies in vitro. We have confirmed the meningothelial origin of these cell lines by assessing expression of vimentin and desmoplakin, characteristic markers for meningiomas. Additionally, we have karyotyped these cell lines using array CGH and shown that they represent a spectrum of the genetic diversity seen in primary meningiomas. Thus, these cell lines represent novel cellular reagents for investigating the molecular oncogenesis of meningiomas.

Original languageEnglish (US)
Pages (from-to)113-121
Number of pages9
JournalJournal of Neuro-Oncology
Volume78
Issue number2
DOIs
StatePublished - Jun 2006
Externally publishedYes

Fingerprint

Primary Cell Culture
Meningioma
Cell Line
Telomerase
Desmoplakins
Vimentin
Growth and Development
Oncogenes
Brain Neoplasms
Transfection
Carcinogenesis
Cell Culture Techniques

Keywords

  • Immortalization
  • Meningioma
  • Model systems
  • Senescence
  • Telomerase

ASJC Scopus subject areas

  • Clinical Neurology
  • Cancer Research
  • Oncology
  • Neuroscience(all)

Cite this

Baia, G. S., Slocum, A. L., Hyer, J. D., Misra, A., Sehati, N., VandenBerg, S. R., ... Lal, A. (2006). A genetic strategy to overcome the senescence of primary meningioma cell cultures. Journal of Neuro-Oncology, 78(2), 113-121. https://doi.org/10.1007/s11060-005-9076-y

A genetic strategy to overcome the senescence of primary meningioma cell cultures. / Baia, Gilson S.; Slocum, Alison L.; Hyer, Jeanette D.; Misra, Anjan; Sehati, Nouzhan; VandenBerg, Scott R.; Feuerstein, Burt G.F.; Deen, Dennis F.; McDermott, Michael W.; Lal, Anita.

In: Journal of Neuro-Oncology, Vol. 78, No. 2, 06.2006, p. 113-121.

Research output: Contribution to journalArticle

Baia, GS, Slocum, AL, Hyer, JD, Misra, A, Sehati, N, VandenBerg, SR, Feuerstein, BGF, Deen, DF, McDermott, MW & Lal, A 2006, 'A genetic strategy to overcome the senescence of primary meningioma cell cultures', Journal of Neuro-Oncology, vol. 78, no. 2, pp. 113-121. https://doi.org/10.1007/s11060-005-9076-y
Baia, Gilson S. ; Slocum, Alison L. ; Hyer, Jeanette D. ; Misra, Anjan ; Sehati, Nouzhan ; VandenBerg, Scott R. ; Feuerstein, Burt G.F. ; Deen, Dennis F. ; McDermott, Michael W. ; Lal, Anita. / A genetic strategy to overcome the senescence of primary meningioma cell cultures. In: Journal of Neuro-Oncology. 2006 ; Vol. 78, No. 2. pp. 113-121.
@article{c8c569c9e9b740c19b199cfb30f08430,
title = "A genetic strategy to overcome the senescence of primary meningioma cell cultures",
abstract = "Even though meningiomas are the second most common brain tumor in adults, little is known about the molecular basis of their growth and development. The lack of suitable cell culture model systems is an impediment to this understanding. Most studies on meningiomas rely on primary, early passage cell lines that eventually senesce or a few established cell lines that have been derived from aggressive variants of meningiomas. We have isolated three primary meningioma cell lines that are negative for telomerase activity. We can overcome the senescence of a Grade III derived meningioma cell line by expressing the telomerase catalytic subunit (hTERT), whereas Grade I meningioma cell lines require the expression of the human papillomavirus E6 and E7 oncogenes in conjunction with hTERT. Meningioma cell lines, immortalized in this manner, maintain their pre-transfection morphology and form colonies in vitro. We have confirmed the meningothelial origin of these cell lines by assessing expression of vimentin and desmoplakin, characteristic markers for meningiomas. Additionally, we have karyotyped these cell lines using array CGH and shown that they represent a spectrum of the genetic diversity seen in primary meningiomas. Thus, these cell lines represent novel cellular reagents for investigating the molecular oncogenesis of meningiomas.",
keywords = "Immortalization, Meningioma, Model systems, Senescence, Telomerase",
author = "Baia, {Gilson S.} and Slocum, {Alison L.} and Hyer, {Jeanette D.} and Anjan Misra and Nouzhan Sehati and VandenBerg, {Scott R.} and Feuerstein, {Burt G.F.} and Deen, {Dennis F.} and McDermott, {Michael W.} and Anita Lal",
year = "2006",
month = "6",
doi = "10.1007/s11060-005-9076-y",
language = "English (US)",
volume = "78",
pages = "113--121",
journal = "Journal of Neuro-Oncology",
issn = "0167-594X",
publisher = "Kluwer Academic Publishers",
number = "2",

}

TY - JOUR

T1 - A genetic strategy to overcome the senescence of primary meningioma cell cultures

AU - Baia, Gilson S.

AU - Slocum, Alison L.

AU - Hyer, Jeanette D.

AU - Misra, Anjan

AU - Sehati, Nouzhan

AU - VandenBerg, Scott R.

AU - Feuerstein, Burt G.F.

AU - Deen, Dennis F.

AU - McDermott, Michael W.

AU - Lal, Anita

PY - 2006/6

Y1 - 2006/6

N2 - Even though meningiomas are the second most common brain tumor in adults, little is known about the molecular basis of their growth and development. The lack of suitable cell culture model systems is an impediment to this understanding. Most studies on meningiomas rely on primary, early passage cell lines that eventually senesce or a few established cell lines that have been derived from aggressive variants of meningiomas. We have isolated three primary meningioma cell lines that are negative for telomerase activity. We can overcome the senescence of a Grade III derived meningioma cell line by expressing the telomerase catalytic subunit (hTERT), whereas Grade I meningioma cell lines require the expression of the human papillomavirus E6 and E7 oncogenes in conjunction with hTERT. Meningioma cell lines, immortalized in this manner, maintain their pre-transfection morphology and form colonies in vitro. We have confirmed the meningothelial origin of these cell lines by assessing expression of vimentin and desmoplakin, characteristic markers for meningiomas. Additionally, we have karyotyped these cell lines using array CGH and shown that they represent a spectrum of the genetic diversity seen in primary meningiomas. Thus, these cell lines represent novel cellular reagents for investigating the molecular oncogenesis of meningiomas.

AB - Even though meningiomas are the second most common brain tumor in adults, little is known about the molecular basis of their growth and development. The lack of suitable cell culture model systems is an impediment to this understanding. Most studies on meningiomas rely on primary, early passage cell lines that eventually senesce or a few established cell lines that have been derived from aggressive variants of meningiomas. We have isolated three primary meningioma cell lines that are negative for telomerase activity. We can overcome the senescence of a Grade III derived meningioma cell line by expressing the telomerase catalytic subunit (hTERT), whereas Grade I meningioma cell lines require the expression of the human papillomavirus E6 and E7 oncogenes in conjunction with hTERT. Meningioma cell lines, immortalized in this manner, maintain their pre-transfection morphology and form colonies in vitro. We have confirmed the meningothelial origin of these cell lines by assessing expression of vimentin and desmoplakin, characteristic markers for meningiomas. Additionally, we have karyotyped these cell lines using array CGH and shown that they represent a spectrum of the genetic diversity seen in primary meningiomas. Thus, these cell lines represent novel cellular reagents for investigating the molecular oncogenesis of meningiomas.

KW - Immortalization

KW - Meningioma

KW - Model systems

KW - Senescence

KW - Telomerase

UR - http://www.scopus.com/inward/record.url?scp=33748146474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748146474&partnerID=8YFLogxK

U2 - 10.1007/s11060-005-9076-y

DO - 10.1007/s11060-005-9076-y

M3 - Article

C2 - 16554968

AN - SCOPUS:33748146474

VL - 78

SP - 113

EP - 121

JO - Journal of Neuro-Oncology

JF - Journal of Neuro-Oncology

SN - 0167-594X

IS - 2

ER -