A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate

Shawn W. Foley, Sager J. Gosai, Dongxue Wang, Nur Selamoglu, Amelia C. Sollitti, Tino Köster, Alexander Steffen, Eric Lyons, Fevzi Daldal, Benjamin A. Garcia, Dorothee Staiger, Roger B. Deal, Brian D. Gregory

Research output: Research - peer-reviewArticle

  • 2 Citations

Abstract

The Arabidopsis thaliana root epidermis is comprised of two cell types, hair and nonhair cells, which differentiate from the same precursor. Although the transcriptional programs regulating these events are well studied, post-transcriptional factors functioning in this cell fate decision are mostly unknown. Here, we globally identify RNA-protein interactions and RNA secondary structure in hair and nonhair cell nuclei. This analysis reveals distinct structural and protein binding patterns across both transcriptomes, allowing identification of differential RNA binding protein (RBP) recognition sites. Using these sequences, we identify two RBPs that regulate hair cell development. Specifically, we find that SERRATE functions in a microRNA-dependent manner to inhibit hair cell fate, while also terminating growth of root hairs mostly independent of microRNA biogenesis. In addition, we show that GLYCINE-RICH PROTEIN 8 promotes hair cell fate while alleviating phosphate starvation stress. In total, this global analysis reveals post-transcriptional regulators of plant root epidermal cell fate.

LanguageEnglish (US)
Pages204-220.e5
JournalDevelopmental Cell
Volume41
Issue number2
DOIs
StatePublished - Apr 24 2017

Fingerprint

RNA
Proteins
MicroRNAs
Plant Roots
RNA-Binding Proteins
Starvation
Cell Nucleus
Transcriptome
Arabidopsis
Protein Binding
Epidermis
Phosphates
Growth

Keywords

  • phosphate starvation response
  • plant development
  • post-transcriptional regulation
  • RNA binding proteins
  • RNA biology
  • RNA secondary structure
  • root hairs

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Foley, S. W., Gosai, S. J., Wang, D., Selamoglu, N., Sollitti, A. C., Köster, T., ... Gregory, B. D. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate. Developmental Cell, 41(2), 204-220.e5. DOI: 10.1016/j.devcel.2017.03.018

A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate. / Foley, Shawn W.; Gosai, Sager J.; Wang, Dongxue; Selamoglu, Nur; Sollitti, Amelia C.; Köster, Tino; Steffen, Alexander; Lyons, Eric; Daldal, Fevzi; Garcia, Benjamin A.; Staiger, Dorothee; Deal, Roger B.; Gregory, Brian D.

In: Developmental Cell, Vol. 41, No. 2, 24.04.2017, p. 204-220.e5.

Research output: Research - peer-reviewArticle

Foley, SW, Gosai, SJ, Wang, D, Selamoglu, N, Sollitti, AC, Köster, T, Steffen, A, Lyons, E, Daldal, F, Garcia, BA, Staiger, D, Deal, RB & Gregory, BD 2017, 'A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate' Developmental Cell, vol 41, no. 2, pp. 204-220.e5. DOI: 10.1016/j.devcel.2017.03.018
Foley SW, Gosai SJ, Wang D, Selamoglu N, Sollitti AC, Köster T et al. A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate. Developmental Cell. 2017 Apr 24;41(2):204-220.e5. Available from, DOI: 10.1016/j.devcel.2017.03.018
Foley, Shawn W. ; Gosai, Sager J. ; Wang, Dongxue ; Selamoglu, Nur ; Sollitti, Amelia C. ; Köster, Tino ; Steffen, Alexander ; Lyons, Eric ; Daldal, Fevzi ; Garcia, Benjamin A. ; Staiger, Dorothee ; Deal, Roger B. ; Gregory, Brian D./ A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate. In: Developmental Cell. 2017 ; Vol. 41, No. 2. pp. 204-220.e5
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