A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate

Shawn W. Foley, Sager J. Gosai, Dongxue Wang, Nur Selamoglu, Amelia C. Sollitti, Tino Köster, Alexander Steffen, Eric H Lyons, Fevzi Daldal, Benjamin A. Garcia, Dorothee Staiger, Roger B. Deal, Brian D. Gregory

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The Arabidopsis thaliana root epidermis is comprised of two cell types, hair and nonhair cells, which differentiate from the same precursor. Although the transcriptional programs regulating these events are well studied, post-transcriptional factors functioning in this cell fate decision are mostly unknown. Here, we globally identify RNA-protein interactions and RNA secondary structure in hair and nonhair cell nuclei. This analysis reveals distinct structural and protein binding patterns across both transcriptomes, allowing identification of differential RNA binding protein (RBP) recognition sites. Using these sequences, we identify two RBPs that regulate hair cell development. Specifically, we find that SERRATE functions in a microRNA-dependent manner to inhibit hair cell fate, while also terminating growth of root hairs mostly independent of microRNA biogenesis. In addition, we show that GLYCINE-RICH PROTEIN 8 promotes hair cell fate while alleviating phosphate starvation stress. In total, this global analysis reveals post-transcriptional regulators of plant root epidermal cell fate.

Original languageEnglish (US)
Pages (from-to)204-220.e5
JournalDevelopmental Cell
Volume41
Issue number2
DOIs
StatePublished - Apr 24 2017

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Keywords

  • phosphate starvation response
  • plant development
  • post-transcriptional regulation
  • RNA binding proteins
  • RNA biology
  • RNA secondary structure
  • root hairs

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Foley, S. W., Gosai, S. J., Wang, D., Selamoglu, N., Sollitti, A. C., Köster, T., Steffen, A., Lyons, E. H., Daldal, F., Garcia, B. A., Staiger, D., Deal, R. B., & Gregory, B. D. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate. Developmental Cell, 41(2), 204-220.e5. https://doi.org/10.1016/j.devcel.2017.03.018