A gradient-free method for the purification of infective dengue virus for protein-level investigations

Stephanie M. Jensen, Celina T. Nguyen, John C Jewett

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free ultracentrifugation followed by selective virion precipitation. This protocol allows for the separation of DENV from contaminating proteins - including intact C6/36 densovirus, for the production of infective virus at high concentration for protein-level analysis.

Original languageEnglish (US)
Pages (from-to)125-130
Number of pages6
JournalJournal of Virological Methods
Volume235
DOIs
StatePublished - Sep 1 2016

Keywords

  • Aedes albopictus
  • Dengue virus
  • Densovirus
  • Infectivity
  • Precipitation
  • Purification

ASJC Scopus subject areas

  • Virology

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