A high-performance microsystem for isolating viable circulating tumor cells

X. J. Zheng, L. S L Cheung, Joyce Schroeder, L. Jiang, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The attachment and detachment of target cancer cells from homogeneous and binary mixtures in antibody-functionalized microchannels have been studied experimentally. Under the same intermediate flow rate, the attachment rate was found to be higher, and detachment flow rate was lower, for cell lines expressing the target receptor at a higher level. For cells that do not express the target receptor, the attachment rate was much lower but did not diminish, due to non-specific binding, and the detachment rate was much higher. The bio-functional microfluidic system performance in selectively isolating target cells from binary mixtures is quantitatively characterized. While the system sensitivity is typically very high, almost 100%, the specificity is lower than 90%. Applying a unique flow scheme of a slow flow rate, for maximum capture of target cells, followed by a faster flow rate, for maximum removal of non-target cells, the specificity is enhanced to levels above 95%, even for mixtures with target cells present at 11,000 relative concentration ratio.

Original languageEnglish (US)
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages226-229
Number of pages4
DOIs
StatePublished - 2011
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: Jun 5 2011Jun 9 2011

Other

Other2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CountryChina
CityBeijing
Period6/5/116/9/11

Fingerprint

Microsystems
Tumors
Cells
Flow rate
Binary mixtures
Microchannels
Microfluidics
Antibodies

Keywords

  • cell isolation
  • Circulating tumor cells
  • Microchannels
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Zheng, X. J., Cheung, L. S. L., Schroeder, J., Jiang, L., & Zohar, Y. (2011). A high-performance microsystem for isolating viable circulating tumor cells. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 226-229). [5969382] https://doi.org/10.1109/TRANSDUCERS.2011.5969382

A high-performance microsystem for isolating viable circulating tumor cells. / Zheng, X. J.; Cheung, L. S L; Schroeder, Joyce; Jiang, L.; Zohar, Yitshak.

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 226-229 5969382.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zheng, XJ, Cheung, LSL, Schroeder, J, Jiang, L & Zohar, Y 2011, A high-performance microsystem for isolating viable circulating tumor cells. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969382, pp. 226-229, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 6/5/11. https://doi.org/10.1109/TRANSDUCERS.2011.5969382
Zheng XJ, Cheung LSL, Schroeder J, Jiang L, Zohar Y. A high-performance microsystem for isolating viable circulating tumor cells. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 226-229. 5969382 https://doi.org/10.1109/TRANSDUCERS.2011.5969382
Zheng, X. J. ; Cheung, L. S L ; Schroeder, Joyce ; Jiang, L. ; Zohar, Yitshak. / A high-performance microsystem for isolating viable circulating tumor cells. 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. pp. 226-229
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