A highly potent agonist to protease-activated receptor-2 reveals apical activation of the airway epithelium resulting in Ca2+-regulated ion conductance

Cara L. Sherwood, Michael O. Daines, Theodore J. Price, Josef Vagner, Scott Boitano

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The airway epithelium provides a barrier that separates inhaled air and its various particulates from the underlying tissues. It provides key physiological functions in both sensing the environment and initiating appropriate innate immune defenses to protect the lung. Protease-activated receptor-2 (PAR2) is expressed both apically and basolaterally throughout the airway epithelium. One consequence of basolateral PAR2 activation is the rapid, Ca2+-dependent ion flux that favors secretion in the normally absorptive airway epithelium. However, roles for apically expressed PAR2 activation have not been demonstrated, in part due to the lack of specific, high-potency PAR2 ligands. In the present study, we used the newly developed PAR2 ligand 2at-LIGRLO(PEG3-Pam)-NH2 in combination with well-differentiated, primary cultured airway epithelial cells from wild-type and PAR2−/− mice to examine the physiological role of PAR2 in the conducting airway after apical activation. Using digital imaging microscopy of intracellular Ca2+concentration changes, we verified ligand potency on PAR2 in primary cultured airway cells. Examination of airway epithelial tissue in an Ussing chamber showed that apical activation of PAR2 by 2at-LIGRLO(PEG3-Pam)-NH2 resulted in a transient decrease in transepithelial resistance that was due to increased apical ion efflux. We determined pharmacologically that this increase in ion conductance was through Ca2+-activated Cl and large-conductance K+ channels that were blocked with a Ca2+-activated Cl channel inhibitor and clotrimazole, respectively. Stimulation of Cl efflux via PAR2 activation at the airway epithelial surface can increase airway surface liquid that would aid in clearing the airway of noxious inhaled agents.

Original languageEnglish (US)
Pages (from-to)C718-C726
JournalAmerican Journal of Physiology - Cell Physiology
Volume307
Issue number8
DOIs
StatePublished - Oct 15 2014

Keywords

  • Allergic asthma
  • Asthmagen
  • Ion conductance
  • Lipidated agonist
  • Protease-activated receptor-2

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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