A histological variant of white spot syndrome virus (WSSV) from the Kingdom of Saudi Arabia

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Abstract

White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp. The major targets of WSSV infection are tissues of ectodermal and mesodermal embryonic origin, predominantly the cuticular epithelium and subcuticular connective tissues. Recently, we discovered a WSSV variant in Penaeus indicus that heavily infects the subcuticular connective tissue, with very slight indications in the cuticular epithelium. The variant was also unusual in that WSSV accumulations were found in the interstitial spaces of both the subcuticular connective tissue and the lymphoid organ. This WSSV variant was confirmed through immunohistochemistry with an anti-WSSV VP28 monoclonal antibody, and also by in situ hybridization with a VP28 DNA probe. By in situ hybridization, shrimp with variant and typical histology were shown a deletion in ORF94, which is characteristic of a new type of WSSV found in Saudi Arabia; apparently, the loss of this ORF is not associated with the variant's reduced capability of infecting the cuticular epithelium cells.

Original languageEnglish (US)
Pages (from-to)82-85
Number of pages4
JournalJournal of Invertebrate Pathology
Volume113
Issue number1
DOIs
StateAccepted/In press - May 2013

Fingerprint

White spot syndrome virus
Saudi Arabia
virus
connective tissues
epithelium
in situ hybridization
Fenneropenaeus indicus
Penaeidae
DNA probes
histology
antibody
immunohistochemistry
open reading frames
monoclonal antibodies
shrimp
probe
DNA
tissue
infection

Keywords

  • Histology variant
  • Immunohistochemistry
  • In situ hybridization
  • Penaeid shrimp
  • White spot syndrome virus
  • WSSV

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "A histological variant of white spot syndrome virus (WSSV) from the Kingdom of Saudi Arabia",
abstract = "White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp. The major targets of WSSV infection are tissues of ectodermal and mesodermal embryonic origin, predominantly the cuticular epithelium and subcuticular connective tissues. Recently, we discovered a WSSV variant in Penaeus indicus that heavily infects the subcuticular connective tissue, with very slight indications in the cuticular epithelium. The variant was also unusual in that WSSV accumulations were found in the interstitial spaces of both the subcuticular connective tissue and the lymphoid organ. This WSSV variant was confirmed through immunohistochemistry with an anti-WSSV VP28 monoclonal antibody, and also by in situ hybridization with a VP28 DNA probe. By in situ hybridization, shrimp with variant and typical histology were shown a deletion in ORF94, which is characteristic of a new type of WSSV found in Saudi Arabia; apparently, the loss of this ORF is not associated with the variant's reduced capability of infecting the cuticular epithelium cells.",
keywords = "Histology variant, Immunohistochemistry, In situ hybridization, Penaeid shrimp, White spot syndrome virus, WSSV",
author = "Feng-Jyu Tang-Nelson and Pantoja-Morales, {Carlos R} and Redman, {Rita M.} and Lightner, {Donald V}",
year = "2013",
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AU - Tang-Nelson, Feng-Jyu

AU - Pantoja-Morales, Carlos R

AU - Redman, Rita M.

AU - Lightner, Donald V

PY - 2013/5

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N2 - White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp. The major targets of WSSV infection are tissues of ectodermal and mesodermal embryonic origin, predominantly the cuticular epithelium and subcuticular connective tissues. Recently, we discovered a WSSV variant in Penaeus indicus that heavily infects the subcuticular connective tissue, with very slight indications in the cuticular epithelium. The variant was also unusual in that WSSV accumulations were found in the interstitial spaces of both the subcuticular connective tissue and the lymphoid organ. This WSSV variant was confirmed through immunohistochemistry with an anti-WSSV VP28 monoclonal antibody, and also by in situ hybridization with a VP28 DNA probe. By in situ hybridization, shrimp with variant and typical histology were shown a deletion in ORF94, which is characteristic of a new type of WSSV found in Saudi Arabia; apparently, the loss of this ORF is not associated with the variant's reduced capability of infecting the cuticular epithelium cells.

AB - White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp. The major targets of WSSV infection are tissues of ectodermal and mesodermal embryonic origin, predominantly the cuticular epithelium and subcuticular connective tissues. Recently, we discovered a WSSV variant in Penaeus indicus that heavily infects the subcuticular connective tissue, with very slight indications in the cuticular epithelium. The variant was also unusual in that WSSV accumulations were found in the interstitial spaces of both the subcuticular connective tissue and the lymphoid organ. This WSSV variant was confirmed through immunohistochemistry with an anti-WSSV VP28 monoclonal antibody, and also by in situ hybridization with a VP28 DNA probe. By in situ hybridization, shrimp with variant and typical histology were shown a deletion in ORF94, which is characteristic of a new type of WSSV found in Saudi Arabia; apparently, the loss of this ORF is not associated with the variant's reduced capability of infecting the cuticular epithelium cells.

KW - Histology variant

KW - Immunohistochemistry

KW - In situ hybridization

KW - Penaeid shrimp

KW - White spot syndrome virus

KW - WSSV

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