A hybrid electrokinetic bioprocessor for static and dynamic pathogen manipulation in physiological samples

Jian Gao, Mandy L.Y. Sin, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a hybrid electrokinetic bioprocessor for label-free manipulation of bacterial pathogens in physiological samples toward lab-on-a-chip applications. While most electrokinetic techniques only function effectively in low-conductivity buffers, hybrid electrokinetics enables operation in high-conductivity samples (∼1 S/m). We tackle the major technical hurdle of electrode instability using a Ti-Au-Ti sandwich configuration for manipulating high conductivity samples. The technique combines short-range electrophoresis and dielectrophoresis, and long-range AC electrothermal flow to dramatically improve its effectiveness. The device is the first AC electrokinetic static/dynamic concentrator that can work directly with physiological fluids, such as urine and buffy coats. We show that uropathogenic bacteria and biowarfare agents can be concentrated for over 3 orders of magnitude.

Original languageEnglish (US)
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages270-273
Number of pages4
DOIs
StatePublished - Sep 1 2011
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: Jun 5 2011Jun 9 2011

Publication series

Name2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Other

Other2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CountryChina
CityBeijing
Period6/5/116/9/11

Keywords

  • Hybrid electrokinetics
  • bacteria pathogens
  • high-conductivity
  • pre-concentration

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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