A hybrid electrokinetic bioprocessor for static and dynamic pathogen manipulation in physiological samples

Jian Gao, Mandy L Y Sin, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a hybrid electrokinetic bioprocessor for label-free manipulation of bacterial pathogens in physiological samples toward lab-on-a-chip applications. While most electrokinetic techniques only function effectively in low-conductivity buffers, hybrid electrokinetics enables operation in high-conductivity samples (∼1 S/m). We tackle the major technical hurdle of electrode instability using a Ti-Au-Ti sandwich configuration for manipulating high conductivity samples. The technique combines short-range electrophoresis and dielectrophoresis, and long-range AC electrothermal flow to dramatically improve its effectiveness. The device is the first AC electrokinetic static/dynamic concentrator that can work directly with physiological fluids, such as urine and buffy coats. We show that uropathogenic bacteria and biowarfare agents can be concentrated for over 3 orders of magnitude.

Original languageEnglish (US)
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages270-273
Number of pages4
DOIs
StatePublished - 2011
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: Jun 5 2011Jun 9 2011

Other

Other2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CountryChina
CityBeijing
Period6/5/116/9/11

Fingerprint

Pathogens
Electrophoresis
Lab-on-a-chip
Labels
Bacteria
Electrodes
Fluids

Keywords

  • bacteria pathogens
  • high-conductivity
  • Hybrid electrokinetics
  • pre-concentration

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Gao, J., Sin, M. L. Y., & Wong, P. K. (2011). A hybrid electrokinetic bioprocessor for static and dynamic pathogen manipulation in physiological samples. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 270-273). [5969403] https://doi.org/10.1109/TRANSDUCERS.2011.5969403

A hybrid electrokinetic bioprocessor for static and dynamic pathogen manipulation in physiological samples. / Gao, Jian; Sin, Mandy L Y; Wong, Pak Kin.

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 270-273 5969403.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gao, J, Sin, MLY & Wong, PK 2011, A hybrid electrokinetic bioprocessor for static and dynamic pathogen manipulation in physiological samples. in 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11., 5969403, pp. 270-273, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, Beijing, China, 6/5/11. https://doi.org/10.1109/TRANSDUCERS.2011.5969403
Gao J, Sin MLY, Wong PK. A hybrid electrokinetic bioprocessor for static and dynamic pathogen manipulation in physiological samples. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. p. 270-273. 5969403 https://doi.org/10.1109/TRANSDUCERS.2011.5969403
Gao, Jian ; Sin, Mandy L Y ; Wong, Pak Kin. / A hybrid electrokinetic bioprocessor for static and dynamic pathogen manipulation in physiological samples. 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11. 2011. pp. 270-273
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