A Karyometric Approach to the Characterization of Atypical Endometrial Hyperplasia with and Without Co-occurring Adenocarcinoma

Francisco A.R. Garcia, John R. Davis, David S. Alberts, Yun Liu, Deborah Thompson, Peter H. Bartels

Research output: Contribution to journalArticle

7 Scopus citations


OBJECTIVE: To develop a karyometric image analysis approach to distinguishing atypical endometrial hyperplasia with and without co-occurring adenocarcinoma. STUDY DESIGN: Six cases of atypical hyperplasia without and 6 cases with co-occurring adenocarcinoma, 4 cases of normal endometrium and 3 cases of adenocar cinoma were identified. From each case 100 nuclei were measured in representative diagnostic areas identified by an experienced pathologist. Discriminant analyses were performed. An unsupervised learning algorithm was applied to define and characterize different nuclear phenotypes, and those data were used to identify cases with cooccurring adenocarcinoma. RESULTS: Discriminant analysis showed that nuclei from atypical hyperplasia and atypical hyperplasia with co-occurring adenocarcinoma are statistically different. The unsupervised learning algorithm revealed differences in nuclear subpopulations that can be used to correctly identify an estimated 85% of individual cases. CONCLUSION: Nuclei from atypical hyperplasia without and with co-occurring adenocarcinoma have statistically different karyometric characteristics that may facilitate case classification.

Original languageEnglish (US)
Pages (from-to)339-346
Number of pages8
JournalAnalytical and Quantitative Cytology and Histology
Issue number6
StatePublished - Dec 1 2003



  • Adenocarcinoma
  • Endometrial cancer
  • Endometrial diseases
  • Hyperplasia
  • Karyometry

ASJC Scopus subject areas

  • Anatomy
  • Histology

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