A live bioprobe for studying diatom-surface interactions

Fernando Terán Arce, Recep Avci, Iwona B. Beech, Keith E. Cooksey, Barbara Wigglesworth-Cooksey

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


Atomic force microscopy has been employed to compare the adhesion of Navicula species I diatoms to surfaces of a hydrophobic elastomer, Intersleek, and a hydrophilic mineral, mica. This was accomplished using tipless atomic force microscopy cantilevers functionalized with live diatom cells. Both surfaces were tested with the same diatom bioprobe. Force versus distance curves generated during these experiments revealed comparable cell adhesion strengths on Intersleek and mica, indicating that Navicula diatoms secrete extracellular polymeric substances with hydrophobic and hydrophilic properties. A statistical analysis of force curves was carried out and the average values of works of detachment of a diatom from Intersleek and mica surfaces were determined.

Original languageEnglish (US)
Pages (from-to)4284-4297
Number of pages14
JournalBiophysical Journal
Issue number6
StatePublished - Dec 2004
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics


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