A map of the distal region of the long arm of human chromosome 21 constructed by radiation hybrid mapping and pulsed-field gel electrophoresis

Margit Burmeister, Suwon Kim, E. Roydon Price, Titia de Lange, Umadevi Tantravahi, Richard M. Myers, David R. Cox

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

We have used radiation hybrid (RH) mapping and pulsedfield gel electrophoresis (PFGE) to determine the order and positions of 28 DNA markers from the distal region of the long arm of human chromosome 21. The maps generated by these two methods are in good agreement. This study, combined with that of D. R. Cox et al. (1990, Science 250:245-250), results in an RH map that covers the long arm of chromosome 21 (21q). We have used a subtelomeric probe to show that our map includes the telomere and have identified single-copy genes and markers within 200 kbp of the telomere. Comparison of the physical and RH maps with genetic linkage maps shows "hot spots" of meiotic recombination in the distal region, one of which is close to the telomere, in agreement with previous cytogenetic observations of increased recombination frequency near telomeres.

Original languageEnglish (US)
Pages (from-to)19-30
Number of pages12
JournalGenomics
Volume9
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

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Radiation Hybrid Mapping
Chromosomes, Human, Pair 21
Pulsed Field Gel Electrophoresis
Telomere
Human Chromosomes
Genetic Recombination
Genetic Linkage
Genetic Markers
Cytogenetics
Electrophoresis
Gels
Genes

ASJC Scopus subject areas

  • Genetics

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A map of the distal region of the long arm of human chromosome 21 constructed by radiation hybrid mapping and pulsed-field gel electrophoresis. / Burmeister, Margit; Kim, Suwon; Price, E. Roydon; Lange, Titia de; Tantravahi, Umadevi; Myers, Richard M.; Cox, David R.

In: Genomics, Vol. 9, No. 1, 1991, p. 19-30.

Research output: Contribution to journalArticle

Burmeister, Margit ; Kim, Suwon ; Price, E. Roydon ; Lange, Titia de ; Tantravahi, Umadevi ; Myers, Richard M. ; Cox, David R. / A map of the distal region of the long arm of human chromosome 21 constructed by radiation hybrid mapping and pulsed-field gel electrophoresis. In: Genomics. 1991 ; Vol. 9, No. 1. pp. 19-30.
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