A mechanism for acetylcholine receptor clustering distinct from agrin signaling

Wade A. Grow, Michael Ferns, Herman Gordon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Acetylcholine receptors (AChRs) and other postsynaptic molecules cluster spontaneously on cultured C2 myotubes. The frequency of clustering is enhanced by neural agrin, neuraminidase, or calcium through a signaling pathway which includes tyrosine phosphorylation of a muscle-specific kinase (MuSK) and the AChR β-subunit Vicia villosa agglutinin (VVA) lectin, previously shown to potentiate agrin-induced clustering on C2 myotubes, is shown here to also potentiate neuraminidase- and calcium-induced clustering of AChRs, while having no effect on the level of tyrosine phosphorylation of MuSK or the AChR β-subunit. We propose that VVA lectin increases the frequency of AChR clustering through a mechanism that is distinct from agrin signaling, and that may involve α-dystroglycan. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish (US)
Pages (from-to)435-443
Number of pages9
JournalDevelopmental Neuroscience
Volume21
Issue number6
StatePublished - 2000

Fingerprint

Agrin
Cholinergic Receptors
Cluster Analysis
Skeletal Muscle Fibers
Neuraminidase
Tyrosine
Phosphorylation
Dystroglycans
Calcium
Muscles
Vicia lectins

Keywords

  • Acetylcholine receptor
  • Agrin
  • Laminin
  • Myotubes
  • Neuraminidase
  • Sialic acid
  • Vicia villosa agglutinin lectin
  • α-Dystroglycan

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A mechanism for acetylcholine receptor clustering distinct from agrin signaling. / Grow, Wade A.; Ferns, Michael; Gordon, Herman.

In: Developmental Neuroscience, Vol. 21, No. 6, 2000, p. 435-443.

Research output: Contribution to journalArticle

@article{67c0dcb4caf44a1a9157e5b6b39bec00,
title = "A mechanism for acetylcholine receptor clustering distinct from agrin signaling",
abstract = "Acetylcholine receptors (AChRs) and other postsynaptic molecules cluster spontaneously on cultured C2 myotubes. The frequency of clustering is enhanced by neural agrin, neuraminidase, or calcium through a signaling pathway which includes tyrosine phosphorylation of a muscle-specific kinase (MuSK) and the AChR β-subunit Vicia villosa agglutinin (VVA) lectin, previously shown to potentiate agrin-induced clustering on C2 myotubes, is shown here to also potentiate neuraminidase- and calcium-induced clustering of AChRs, while having no effect on the level of tyrosine phosphorylation of MuSK or the AChR β-subunit. We propose that VVA lectin increases the frequency of AChR clustering through a mechanism that is distinct from agrin signaling, and that may involve α-dystroglycan. Copyright (C) 2000 S. Karger AG, Basel.",
keywords = "Acetylcholine receptor, Agrin, Laminin, Myotubes, Neuraminidase, Sialic acid, Vicia villosa agglutinin lectin, α-Dystroglycan",
author = "Grow, {Wade A.} and Michael Ferns and Herman Gordon",
year = "2000",
language = "English (US)",
volume = "21",
pages = "435--443",
journal = "Developmental Neuroscience",
issn = "0378-5866",
publisher = "S. Karger AG",
number = "6",

}

TY - JOUR

T1 - A mechanism for acetylcholine receptor clustering distinct from agrin signaling

AU - Grow, Wade A.

AU - Ferns, Michael

AU - Gordon, Herman

PY - 2000

Y1 - 2000

N2 - Acetylcholine receptors (AChRs) and other postsynaptic molecules cluster spontaneously on cultured C2 myotubes. The frequency of clustering is enhanced by neural agrin, neuraminidase, or calcium through a signaling pathway which includes tyrosine phosphorylation of a muscle-specific kinase (MuSK) and the AChR β-subunit Vicia villosa agglutinin (VVA) lectin, previously shown to potentiate agrin-induced clustering on C2 myotubes, is shown here to also potentiate neuraminidase- and calcium-induced clustering of AChRs, while having no effect on the level of tyrosine phosphorylation of MuSK or the AChR β-subunit. We propose that VVA lectin increases the frequency of AChR clustering through a mechanism that is distinct from agrin signaling, and that may involve α-dystroglycan. Copyright (C) 2000 S. Karger AG, Basel.

AB - Acetylcholine receptors (AChRs) and other postsynaptic molecules cluster spontaneously on cultured C2 myotubes. The frequency of clustering is enhanced by neural agrin, neuraminidase, or calcium through a signaling pathway which includes tyrosine phosphorylation of a muscle-specific kinase (MuSK) and the AChR β-subunit Vicia villosa agglutinin (VVA) lectin, previously shown to potentiate agrin-induced clustering on C2 myotubes, is shown here to also potentiate neuraminidase- and calcium-induced clustering of AChRs, while having no effect on the level of tyrosine phosphorylation of MuSK or the AChR β-subunit. We propose that VVA lectin increases the frequency of AChR clustering through a mechanism that is distinct from agrin signaling, and that may involve α-dystroglycan. Copyright (C) 2000 S. Karger AG, Basel.

KW - Acetylcholine receptor

KW - Agrin

KW - Laminin

KW - Myotubes

KW - Neuraminidase

KW - Sialic acid

KW - Vicia villosa agglutinin lectin

KW - α-Dystroglycan

UR - http://www.scopus.com/inward/record.url?scp=0041382568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041382568&partnerID=8YFLogxK

M3 - Article

C2 - 10640862

AN - SCOPUS:0041382568

VL - 21

SP - 435

EP - 443

JO - Developmental Neuroscience

JF - Developmental Neuroscience

SN - 0378-5866

IS - 6

ER -