A mechanostimulation system for revealing intercellular calcium communication in HUVEC networks

Michael Junkin, Yi Lu, Juexuan Long, Pierre A Deymier, James B. Hoying, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a mechanostimulation system for studying mechanically induced intercellular calcium signaling in networks of human umbilical vein endothelial cells (HUVECs). By incorporating a capacitive (comb drive) force probe and plasma lithography cell patterning, the roles of biophysical factors, including force, duration, and network architecture, in calcium intercellular communication can be investigated systematically. Particularly, we observed cancellation of calcium waves in linear networks and bi-directional splitting in cross junctions. The effects of key biophysical factors on intercellular calcium wave propagation were studied. These results demonstrate the applicability of the mechanostimulation system in studying intercellular calcium signaling and reveal the robustness of calcium signaling in HUVEC networks, which mimics the vasculature.

Original languageEnglish (US)
Title of host publicationIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Pages92-95
Number of pages4
DOIs
StatePublished - 2012
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: Nov 4 2012Nov 7 2012

Other

Other6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
CountryThailand
CityBangkok
Period11/4/1211/7/12

Fingerprint

Calcium Signaling
Endothelial cells
Human Umbilical Vein Endothelial Cells
Calcium
Communication
Comb and Wattles
Linear networks
Plasma Cells
Network architecture
Wave propagation
Lithography
Plasmas

Keywords

  • Calcium signaling
  • Endothelial networks
  • HUVEC
  • Intercellular Communications

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

Cite this

Junkin, M., Lu, Y., Long, J., Deymier, P. A., Hoying, J. B., & Wong, P. K. (2012). A mechanostimulation system for revealing intercellular calcium communication in HUVEC networks. In IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED (pp. 92-95). [6509116] https://doi.org/10.1109/NANOMED.2012.6509116

A mechanostimulation system for revealing intercellular calcium communication in HUVEC networks. / Junkin, Michael; Lu, Yi; Long, Juexuan; Deymier, Pierre A; Hoying, James B.; Wong, Pak Kin.

IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. 2012. p. 92-95 6509116.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Junkin, M, Lu, Y, Long, J, Deymier, PA, Hoying, JB & Wong, PK 2012, A mechanostimulation system for revealing intercellular calcium communication in HUVEC networks. in IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED., 6509116, pp. 92-95, 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012, Bangkok, Thailand, 11/4/12. https://doi.org/10.1109/NANOMED.2012.6509116
Junkin M, Lu Y, Long J, Deymier PA, Hoying JB, Wong PK. A mechanostimulation system for revealing intercellular calcium communication in HUVEC networks. In IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. 2012. p. 92-95. 6509116 https://doi.org/10.1109/NANOMED.2012.6509116
Junkin, Michael ; Lu, Yi ; Long, Juexuan ; Deymier, Pierre A ; Hoying, James B. ; Wong, Pak Kin. / A mechanostimulation system for revealing intercellular calcium communication in HUVEC networks. IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. 2012. pp. 92-95
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