A meiotic recombination checkpoint controlled by mitotic checkpoint genes

D. Lydall, Y. Nikolsky, D. K. Bishop, Ted Weinert

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

IN budding yeast, meiotic recombination occurs at about 200 sites per cell and involves DNA double-strand break (DSB) intermediates. Here we provide evidence that a checkpoint control requiring the mitotic DNA-damage checkpoint genes RAD17, RAD24 and MEC1 ensures that meiotic recombination is complete before the first ratiotic division (MI). First, RAD17, RAD24 and MEC1 are required for the meiotic arrest caused by blocking the repair of DSBs with a mutation in the recA homologue DMC1. Second, mec1 and rad24 single routants (DMC1+) appear to undergo MI before all recombination events are complete. Curiously, the mitosis-specific checkpoint gene RAD9 is not required for meiotic arrest of dmc1 mutants. This shows that although mitotic and meiotic control mechanisms are related, they differ significantly. Rad17 and Rad24 proteins may contribute directly to formation of an arrest signal by association with single-strand DNA in mitosis and meiosis.

Original languageEnglish (US)
Pages (from-to)841-844
Number of pages4
JournalNature
Volume383
Issue number6603
StatePublished - 1996

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M Phase Cell Cycle Checkpoints
Genetic Recombination
Mitosis
Genes
Saccharomycetales
Double-Stranded DNA Breaks
Meiosis
DNA Damage
Mutation
DNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Lydall, D., Nikolsky, Y., Bishop, D. K., & Weinert, T. (1996). A meiotic recombination checkpoint controlled by mitotic checkpoint genes. Nature, 383(6603), 841-844.

A meiotic recombination checkpoint controlled by mitotic checkpoint genes. / Lydall, D.; Nikolsky, Y.; Bishop, D. K.; Weinert, Ted.

In: Nature, Vol. 383, No. 6603, 1996, p. 841-844.

Research output: Contribution to journalArticle

Lydall, D, Nikolsky, Y, Bishop, DK & Weinert, T 1996, 'A meiotic recombination checkpoint controlled by mitotic checkpoint genes', Nature, vol. 383, no. 6603, pp. 841-844.
Lydall D, Nikolsky Y, Bishop DK, Weinert T. A meiotic recombination checkpoint controlled by mitotic checkpoint genes. Nature. 1996;383(6603):841-844.
Lydall, D. ; Nikolsky, Y. ; Bishop, D. K. ; Weinert, Ted. / A meiotic recombination checkpoint controlled by mitotic checkpoint genes. In: Nature. 1996 ; Vol. 383, No. 6603. pp. 841-844.
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