A method for determining ascaris viability based on early-to-late stage in-vitro ova development

Bradley W. Schmitz, Jennifer Pearce-Walker, Charles P. Gerba, Ian L. Pepper

Research output: Research - peer-reviewArticle

  • 1 Citations

Abstract

This study suggests a new method for determining the viability of Ascaris spp. ova, based on in-vitro early-to-late stage development of ova. This method includes stages prior to larval development, providing an estimation of potential viability. After application of biosolids onto soil and exposure to 7°C, 22°C, or 37°C for 45 days, ova were microscopically distinguished as viable or non-viable according to progression through development categories. Results were compared to viability estimates from current methods that distinguish viable ova as motile larva. Results suggest conventional techniques underestimate viability, whereas the new method provides a more conservative approach.

LanguageEnglish (US)
Pages275-286
Number of pages12
JournalJournal of Residuals Science and Technology
Volume13
Issue number4
DOIs
StatePublished - 2016

Fingerprint

viability
method
Biosolids
Soils
biosolid
larval development
larva
soil
exposure

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal

Cite this

A method for determining ascaris viability based on early-to-late stage in-vitro ova development. / Schmitz, Bradley W.; Pearce-Walker, Jennifer; Gerba, Charles P.; Pepper, Ian L.

In: Journal of Residuals Science and Technology, Vol. 13, No. 4, 2016, p. 275-286.

Research output: Research - peer-reviewArticle

@article{4a9932724f4145acabade6781140189a,
title = "A method for determining ascaris viability based on early-to-late stage in-vitro ova development",
abstract = "This study suggests a new method for determining the viability of Ascaris spp. ova, based on in-vitro early-to-late stage development of ova. This method includes stages prior to larval development, providing an estimation of potential viability. After application of biosolids onto soil and exposure to 7°C, 22°C, or 37°C for 45 days, ova were microscopically distinguished as viable or non-viable according to progression through development categories. Results were compared to viability estimates from current methods that distinguish viable ova as motile larva. Results suggest conventional techniques underestimate viability, whereas the new method provides a more conservative approach.",
author = "Schmitz, {Bradley W.} and Jennifer Pearce-Walker and Gerba, {Charles P.} and Pepper, {Ian L.}",
year = "2016",
doi = "10.12783/issn.1544-8053/13/4/5",
volume = "13",
pages = "275--286",
journal = "Journal of Residuals Science and Technology",
issn = "1544-8053",
publisher = "DEStech Publications Inc.",
number = "4",

}

TY - JOUR

T1 - A method for determining ascaris viability based on early-to-late stage in-vitro ova development

AU - Schmitz,Bradley W.

AU - Pearce-Walker,Jennifer

AU - Gerba,Charles P.

AU - Pepper,Ian L.

PY - 2016

Y1 - 2016

N2 - This study suggests a new method for determining the viability of Ascaris spp. ova, based on in-vitro early-to-late stage development of ova. This method includes stages prior to larval development, providing an estimation of potential viability. After application of biosolids onto soil and exposure to 7°C, 22°C, or 37°C for 45 days, ova were microscopically distinguished as viable or non-viable according to progression through development categories. Results were compared to viability estimates from current methods that distinguish viable ova as motile larva. Results suggest conventional techniques underestimate viability, whereas the new method provides a more conservative approach.

AB - This study suggests a new method for determining the viability of Ascaris spp. ova, based on in-vitro early-to-late stage development of ova. This method includes stages prior to larval development, providing an estimation of potential viability. After application of biosolids onto soil and exposure to 7°C, 22°C, or 37°C for 45 days, ova were microscopically distinguished as viable or non-viable according to progression through development categories. Results were compared to viability estimates from current methods that distinguish viable ova as motile larva. Results suggest conventional techniques underestimate viability, whereas the new method provides a more conservative approach.

UR - http://www.scopus.com/inward/record.url?scp=84994602818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994602818&partnerID=8YFLogxK

U2 - 10.12783/issn.1544-8053/13/4/5

DO - 10.12783/issn.1544-8053/13/4/5

M3 - Article

VL - 13

SP - 275

EP - 286

JO - Journal of Residuals Science and Technology

T2 - Journal of Residuals Science and Technology

JF - Journal of Residuals Science and Technology

SN - 1544-8053

IS - 4

ER -