A method for the production of CD4+ chronic myelogenous leukemia- specific allogeneic T lymphocytes

Jonathan S. Serody, Mark E. Brecher, Georgette Dent, Stuart A. Bentley, Jeffrey A. Frelinger, Thomas C. Shea

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The graft-versus-leukemia effect is critical to the maintenance of remission in patients transplanted for the treatment of chronic myelogenous leukemia (CML). A pivotal issue in transplantation for CML is whether donor lymphocytes are specific for host tumor or myeloid cells or a subset of the lymphocytes that cause graft-versus-host disease. We have enrolled seven patients in an experimental trial to evaluate the specificity of HLA-matched donor lymphocytes in vitro. We have produced 11 CD4+ cytotoxic and proliferative T-cell clones from five of the donors that only lyse or proliferate to leukemic myeloid cells. These T lymphocytes do not react with interleukin (IL)-2-stimulated blasts, natural killer-sensitive targets, donor neutrophils, or bcr-abl+ EBV-lymphoblastoid cell lines. We show that the addition of the cytokines IL-7 and IL-12 during the production of T-cell clones enhances the recovery of myeloid-specific clones in vitro. Five of the myeloid-specific clones that we produced maintained specificity over 12 weeks in culture. Adoption of this method should allow for the expansion and in vivo testing of CML-specific CD4+ T-cell clones in adoptive immunotherapy.

Original languageEnglish (US)
Pages (from-to)1547-1553
Number of pages7
JournalCancer Research
Volume57
Issue number8
StatePublished - Jan 1 1997
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Serody, J. S., Brecher, M. E., Dent, G., Bentley, S. A., Frelinger, J. A., & Shea, T. C. (1997). A method for the production of CD4+ chronic myelogenous leukemia- specific allogeneic T lymphocytes. Cancer Research, 57(8), 1547-1553.