A method to detect enteroviruses in sewage sludge-amended soil using the PCR

T. M. Straub, I. L. Pepper, M. Abbaszadegan, C. P. Gerba

Research output: Contribution to journalComment/debate

32 Scopus citations

Abstract

PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.

Original languageEnglish (US)
Pages (from-to)1014-1017
Number of pages4
JournalApplied and environmental microbiology
Volume60
Issue number3
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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