A microfluidics-based in vitro model of the gastrointestinal human-microbe interface

Pranjul Shah, Joëlle V. Fritz, Enrico Glaab, Mahesh S. Desai, Kacy Greenhalgh, Audrey Frachet, Magdalena Niegowska, Matthew Estes, Christian Jäger, Carole Seguin-Devaux, Frederic Zenhausern, Paul Wilmes

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.

Original languageEnglish (US)
Article number11535
JournalNature Communications
Volume7
DOIs
StatePublished - May 11 2016

Fingerprint

Lactobacillus rhamnosus
Microfluidics
microorganisms
anaerobes
Coculture Techniques
animal models
molecular interactions
crosstalk
health
Molecular interactions
Crosstalk
Animals
Epithelial Cells
cells
Health
Bacteroides
Microbiota
Causality
In Vitro Techniques
Animal Models

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Shah, P., Fritz, J. V., Glaab, E., Desai, M. S., Greenhalgh, K., Frachet, A., ... Wilmes, P. (2016). A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. Nature Communications, 7, [11535]. https://doi.org/10.1038/ncomms11535

A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. / Shah, Pranjul; Fritz, Joëlle V.; Glaab, Enrico; Desai, Mahesh S.; Greenhalgh, Kacy; Frachet, Audrey; Niegowska, Magdalena; Estes, Matthew; Jäger, Christian; Seguin-Devaux, Carole; Zenhausern, Frederic; Wilmes, Paul.

In: Nature Communications, Vol. 7, 11535, 11.05.2016.

Research output: Contribution to journalArticle

Shah, P, Fritz, JV, Glaab, E, Desai, MS, Greenhalgh, K, Frachet, A, Niegowska, M, Estes, M, Jäger, C, Seguin-Devaux, C, Zenhausern, F & Wilmes, P 2016, 'A microfluidics-based in vitro model of the gastrointestinal human-microbe interface', Nature Communications, vol. 7, 11535. https://doi.org/10.1038/ncomms11535
Shah P, Fritz JV, Glaab E, Desai MS, Greenhalgh K, Frachet A et al. A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. Nature Communications. 2016 May 11;7. 11535. https://doi.org/10.1038/ncomms11535
Shah, Pranjul ; Fritz, Joëlle V. ; Glaab, Enrico ; Desai, Mahesh S. ; Greenhalgh, Kacy ; Frachet, Audrey ; Niegowska, Magdalena ; Estes, Matthew ; Jäger, Christian ; Seguin-Devaux, Carole ; Zenhausern, Frederic ; Wilmes, Paul. / A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. In: Nature Communications. 2016 ; Vol. 7.
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