A miniaturized cyclic PCR device - Modeling and experiments

Chia Fu Chou, R. Changrani, P. Roberts, D. Sadler, J. Burdon, Frederic Zenhausern, S. Lin, A. Mulholland, N. Swami, R. Terbrueggen

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

With the aid of thermal and fluidic modeling using CFDRC ACE + ™, we designed and fabricated the first miniaturized cyclic polymerase chain reaction (PCR) device in low-temperature cofired ceramics. The device comprises of a serpentine channel with different cross-sectional areas in different reactor zones to provide adequate residence time for the melting, annealing, and extension reaction to take place. This is in contrary to the thermal cycling in the batch PCR system. With a flow rate of 15 μl/min, the designed time to complete 30 PCR cycles is less than 40 min, given the total volume of the device 19 μl, provided an internal pump may be implemented to reduce the dead volume. We have demonstrated DNA amplification in this device, using an external peristaltic pump, and the PCR product was used with a DNA bioelectronic sensor chip (Motorola e-Sensors™) for genotyping experiment.

Original languageEnglish (US)
Pages (from-to)921-925
Number of pages5
JournalMicroelectronic Engineering
Volume61-62
DOIs
StatePublished - Jul 2002
Externally publishedYes

Fingerprint

polymerase chain reaction
Polymerase chain reaction
DNA
deoxyribonucleic acid
Experiments
Pumps
pumps
cycles
fluidics
sensors
Sensors
Fluidics
Thermal cycling
Reaction products
reaction products
Amplification
Melting
flow velocity
chips
reactors

Keywords

  • Ceramics
  • Continuous-flow
  • Microfluidics
  • PCR
  • Thermal modeling

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Atomic and Molecular Physics, and Optics

Cite this

Chou, C. F., Changrani, R., Roberts, P., Sadler, D., Burdon, J., Zenhausern, F., ... Terbrueggen, R. (2002). A miniaturized cyclic PCR device - Modeling and experiments. Microelectronic Engineering, 61-62, 921-925. https://doi.org/10.1016/S0167-9317(02)00450-1

A miniaturized cyclic PCR device - Modeling and experiments. / Chou, Chia Fu; Changrani, R.; Roberts, P.; Sadler, D.; Burdon, J.; Zenhausern, Frederic; Lin, S.; Mulholland, A.; Swami, N.; Terbrueggen, R.

In: Microelectronic Engineering, Vol. 61-62, 07.2002, p. 921-925.

Research output: Contribution to journalArticle

Chou, CF, Changrani, R, Roberts, P, Sadler, D, Burdon, J, Zenhausern, F, Lin, S, Mulholland, A, Swami, N & Terbrueggen, R 2002, 'A miniaturized cyclic PCR device - Modeling and experiments', Microelectronic Engineering, vol. 61-62, pp. 921-925. https://doi.org/10.1016/S0167-9317(02)00450-1
Chou, Chia Fu ; Changrani, R. ; Roberts, P. ; Sadler, D. ; Burdon, J. ; Zenhausern, Frederic ; Lin, S. ; Mulholland, A. ; Swami, N. ; Terbrueggen, R. / A miniaturized cyclic PCR device - Modeling and experiments. In: Microelectronic Engineering. 2002 ; Vol. 61-62. pp. 921-925.
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