A modified model of hindlimb osteomyocutaneous flap for the study of tolerance to composite tissue allografts

Larry A. Adamson, Wei Chao Huang, Warren C. Breidenbach, Dina Rahhal, Hong Xu, Yiming Huang, Diane J. Pidwell, Fu Chan Wei, Gordon Tobin, Suzanne T. Ildstad

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Composite tissue allotransplantation (CTA) is the new frontier in transplantation. More than 25 hand allograft transplants have been performed worldwide, and the feasibility has been well established. The classical experimental model of CTA involves rat orthotopic hindlimb transplantation, a time-consuming procedure associated with high mortality and morbidity. We describe a rat heterotopic osteomyocutaneous flap that serves as a nonfunctional CTA, allowing the study of tolerance induction to a highly antigenic vascularized allograft of bone, muscle, and skin while minimizing the morbidity and mortality of full hind limb transplantation. In the present studies, we explored whether establishing chimerism by nonmyeloablative conditioning would induce tolerance to CTA. When compared with the classic hind limb transplantation model, these results demonstrate that our heterotopic hind limb flap is less morbid and as an effective experimental model for the study of CTA tolerance.

Original languageEnglish (US)
Pages (from-to)630-636
Number of pages7
JournalMicrosurgery
Volume27
Issue number7
DOIs
StatePublished - Nov 14 2007
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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    Adamson, L. A., Huang, W. C., Breidenbach, W. C., Rahhal, D., Xu, H., Huang, Y., Pidwell, D. J., Wei, F. C., Tobin, G., & Ildstad, S. T. (2007). A modified model of hindlimb osteomyocutaneous flap for the study of tolerance to composite tissue allografts. Microsurgery, 27(7), 630-636. https://doi.org/10.1002/micr.20414